Sequences from confirmation amplicon


Confirmation sequences for line 737G08 and hit At5g19080 are shown.
Line ID 737G08
Locus At5g19080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6379363
Primers used for PCR 58lm - 8474, show primer details
   
Sequence Name 09-K113981-0022-737-G08C-8409
GenBank Accession LN490268 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K113981-0022-737-G08C-8409
TAACTTGGTATGCACACAGGAGAGTATAAGGTTGATTCAGTTGCTAACTAGCTTAGTAGT
TCCGAACCATTAAATGAACTACAATAACTCCTAAAATCAATCAGTTATGTAACTGAAGCC
TGTATTGTTTGGTTTCTGAACCAATCACATATTCATCACCTAGATCATGTCAAATTTTAA
CCTTTTTCATCCCAAATTGTGGCAACACATAATTCAGAACTGAAACATGAGTAAAGATGT
TTCCTTTTAATCAATCTCGTCCCAGCAAAAACCCTAACTTTCACTGAATAAATGAGTCAT
ATCATGTAAAGTTTGAACCTTTACGATCCAAATTTGAGATTCAGTATAATCTACAAGACC
CCTCTACGCTAATTAGCCAATCTCATTCAAATTCTGGTTCTAAAGTCGGAAACTTGACTC
AAATTTTGGAGTGAAGAAGTTATACCTGCCATCGAATAAGGCATCGAAGACGAAGAAAAC
AAGAT
   
Sequence Name 25-K113982-0022-737-G08C-58lm
GenBank Accession LN497538 [GenBank]
Sequencing primer gene specific primer, 58lm: 5'-TATCTTGTTTCCTTCGTCTTCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K113982-0022-737-G08C-58lm
TCCAAATTTGAGTCAGTTTCCGACTTTAGAACCAGAATTTGAATGAGATTGGCTAATTAG
CGTAGAGGGGTCTTGTAGATTATACTGAATCTCAAATTTGGATCGTAAAGGTTCAAACTT
TACATGATATGACTCATTTATTCAGTGAAAGTTAGGGTTTTTGCTGGGACGAGATTGATT
AAAAGGAAACATCTTTACTCATGTTTCAGTTCTGAATTATGTGTTGCCACAATTTGGGAT
GAAAAAGGTTAAAATTTGACATGATCTAGGTGATGAATATGTGATTGGTTCAGAAACCAA
ACAATACAGGCTTCAGTTACATAACTGATTGATTTTAGGAGTTATTGTAGTTCATTTAAT
GGTTCGGAACTACTAAGCTAGTTAGCAACTGAATCAACCTTATTACTCTCCTGTGTTGCA
TACCAAGTTTAGGTATTAAGATTATCTGACGAGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCAGCA
GCGTTATT
Primers used for PCR 05me - 8474, show primer details
   
Sequence Name 21-K114053-0022-737-G08C-05me
GenBank Accession LN495752 [GenBank]
Sequencing primer gene specific primer, 05me: 5'-ATTATCTTGTTTCCTTCGTCTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K114053-0022-737-G08C-05me
AAATTTGAGTCAGTTTCCGACTTTAGAACCAGAATTTGAATGAGATTGGCTAATTAGCGT
AGAGGGGTCTTGTAGATTATACTGAATCTCAAATTTGGATCGTAAAGGTTCAAACTTTAC
ATGATATGACTCATTTATTCAGTGAAAGTTAGGGTTTTTGCTGGGACGAGATTGATTAAA
AGGAAACATCTTTACTCATGTTTCAGTTCTGAATTATGTGTTGCCACAATTTGGGATGAA
AAAGGTTAAAATTTGACATGATCTAGGTGATGAATATGTGATTGGTTCAGAAACCAAACA
ATACAGGCTTCAGTTACATAACTGATTGATTTTAGGAGTTATTGTAGTTCATTTAATGGT
TCGGAACTACTAAGCTAGTTAGCAACTGAATCAACCTTATTACTCTCCTGTGTTGCATAC
CAAGTTTAGGTATTAAGATTATCTGACGAGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCA
   
Sequence Name 05-K114052-0022-737-G08C-8409
GenBank Accession LN488440 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K114052-0022-737-G08C-8409
TAACTTGGTATGCACACANGAGAGTAATAAGGTTGATTCAGTTGCTAACTAGCTTAGTAG
TTCCGAACCATTAAATGAACTACAATAACTCCTAAAATCAATCAGTTATGTAACTGAAGC
CTGTATTGTTTGGTTTCTGAACCAATCACATATTCATCACCTAGATCATGTCAAATTTTA
ACCTTTTTCATCCCAAATTGTGGCAACACATAATTCAGAACTGAAACATGAGTAAAGATG
TTTCCTTTTAATCAATCTCGTCCCAGCAAAAACCCTAACTTTCACTGAATAAATGAGTCA
TATCATGTAAAGTTTGAACCTTTACGATCCAAATTTGAGATTCAGTATAATCTACAAGAC
CCCTCTACGCTAATTAGCCAATCTCATTCAAATTCTGGTTCTAAAGTCGGAAACTTGACT
CAAATTTTGGAGTGAAGAAGTTATACCTGCCATCGAATAAGGCATCGAAGACGAAGG

Last Updated on 06.12.2018 16:11