Sequences from confirmation amplicon


Confirmation sequences for line 747A04 and hit At2g19170 are shown.
Line ID 747A04
Locus At2g19170 [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8315143
Primers used for PCR MA65 - 8474, show primer details
   
Sequence Name 86-K102508-0022-747-A04C-8409
GenBank Accession LN514642 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>86-K102508-0022-747-A04C-8409
CATTTACATTGACATCATACTCATTGCTCAGTTCTCTATTTCAGCTAGAGGACCCAACAC
CAAAGATTTCAGCTTTCAAGATGCTGATCTTCTTAAACCGGATATTCTTGCACCCGGTTA
TCTCATATGGGCTGCTTGGTGTCCAAATGGAACAGATGAACCCAATTATGTTGGTAAGCA
TTGATAGTACTGAAGCTAGCATGTGCCGAATCTTTTGAATCAGAATTGTGGTCTCTTACT
TTGGGATATTGAGCAGGTGAAGGATTTGCGCTAATATCAGGAACCAGCATGGCTGCACCA
CACATAGCCGGGATAGCTGCGCTTGTGAAGCAGAAGCATCCTCAATGGAGCCCAGCTGCT
ATCAAATCAGCTTTGATGACAACCTCAACAGTCATAGATAGAGCAGGAAGGCTTCTTCAG
GCACAGCAATATTCAGACACAGAAGCTGTAACGCTTGTTAAAGCCACTCCTTTCGATTAT
GGAAGTGGTCATGTCAATCCAAGTGCTGCTTTAGATCCTGGTCTTATCTTTGATGCAGGT
ATTAATTTCTATTTCTCATCAATGATGTACCAAAGATTTTTGATATCTGGTTGATAAGAC
CTATCAATCTTCTCTTGTAGGTTATGAGGACTATCTAGGATTCTTATGCACAACCCCAGG
GATCAGCGCCCACGAGATAAGAAATTAC
   
Sequence Name 86-K102509-0022-747-A04C-MA65
GenBank Accession LN514643 [GenBank]
Sequencing primer gene specific primer, MA65: 5'-GGCCGTGTTTGTGTAATTTCTTAT-3'
Sequence (A. th genome BLAST matches underlined)
>86-K102509-0022-747-A04C-MA65
CTAGATAGTCCTCATAACCTACAAGAGAAGATTGATAGGTCTTATCAACCAGATATCAAA
AATCTTTGGTACATCATTGATGAGAAATAGAAATTAATACCTGCATCAAAGATAAGACCA
GGATCTAAAGCAGCACTTGGATTGACATGACCACTTCCATAATCGAAAGGAGTGGCTTTA
ACAAGCGTTACAGCTTCTGTGTCTGAATATTGCTGTGCCTGAAGAAGCCTTCCTGCTCTA
TCTATGACTGTTGAGGTTGTCATCAAAGCTGATTTGATAGCAGCTGGGCTCCATTGAGGA
TGCTTCTGCTTCACAAGCGCAGCTATCCCGGCTATGTGTGGTGCAGCCATGCTGGTTCCT
GATATTAGCGCAAATCCTTCACCTGCTCAATATCCCAAAGTAAGAGACCACAATTCTGAT
TCAAAAGATTCGGCACATGCTAGCTTCAGTACTATCAATGCTTACCAACATAATTGGGTT
CATCTGTTCCATTTGGACACCAAGCAGCCCATATGAGATAACCGGGTGCAAGAATATCCG
GTTTAAGAAGATCAGCATCTTGAAAGCTGAAATCTTTGGTGTTGGGTCCTCTAGCTGAAA
TAGAGAACTGAGCAATGAGTATGATGTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAATTTATCTA
ATTCGGAAAATCTTATTCGACGTGTCTA

Last Updated on 23.10.2014 13:25