Sequences from confirmation amplicon


Confirmation sequences for line 747G02 and hit At3g14570 are shown.
Line ID 747G02
Locus At3g14570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4893204
Primers used for PCR CS09 - 8474, show primer details
   
Sequence Name 73-K030602-022-747-G02C-CS09
GenBank Accession LN512820 [GenBank]
Sequencing primer gene specific primer, CS09: 5'-CTAATCCCTAGAACACGAATCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K030602-022-747-G02C-CS09
AGCACGAGTTACTCACGGCCAATTCCGGGTCCTCGTGAAGAATCGCCGGAACGTGCTACC
GAGTTCACGAGATTCCTAACTTTTCGGGAACATGTGTCGTCGGAGCCTTTTGATAGCGAG
AGGCTTCCAGCGACATTAGCTTCCGAGATTCAGAGGTTTCTTCGTATTGCTAACTTGGTT
GAGTCTGAGGAACCTCGAATTGCTTATCTATGTGAGTTCACTCTCTCCTCTTCCCTAATT
TTGTAGGTCAATATTTCACTGAGTTTTTCTTCGAAATTCAAGCTTTCTTGTTGAAGAATC
ATACTTGAGTACCAATGTTGAAATTCTGTCGCTGCANGCCGATTTCATG
   
Sequence Name 34-K030602-022-747-G02C-8409
GenBank Accession LN501296 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K030602-022-747-G02C-8409
ATTGAATAATCCTGCAAAGTAAAGTGATGATTTTTTAGAAAATTTCATGACTTATTCAAA
AACTTTCTACTCATCTGTTTGAAGACTCGCCAGTTCAAGCCTTTGAAGAAGAGAAGTTTT
GAACTGCCGTACACCTCTTCCGGTAGAGTTTCTATCCATGTGGTGGGCTATCTCGAAAGC
ATGAAATCGGCCTGCAGCGACAGAATTTCAACATTGGTACTCAAGTATGATTCTTCAACA
AGAAAGCTTGAATTTCGAAGAAAAACTCAGTGAAATATTGACCTACAAAATTAGGGAAGA
AGAGAGAGTGAACTCACATAGATAAGCAATTCGAGGTTCCTCAGACTCAACCAAGTTAGC
AATACGAAGAAACCTCTGAATCTCGGAAGCTAATGTCGCTGGAAGCCTCTCGCTATCAAA
AGGCTCCGACGACACATGTTCCCGAAAAGTTAGGGATCTCGTGAAACTCGTAGCACGTTC
CGGCGAATCTTC

Last Updated on 06.12.2018 16:11