Sequences from confirmation amplicon


Confirmation sequences for line 751B10 and hit At1g65890 are shown.
Line ID 751B10
Locus At1g65890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24514453
Primers used for PCR IW34 - 8474, show primer details
   
Sequence Name 27-K033212-022-751-B10C-IW34
GenBank Accession LN498278 [GenBank]
Sequencing primer gene specific primer, IW34: 5'-CGGTATATGAATAAAATCTTGGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K033212-022-751-B10C-IW34
GAGATGCGGCATGGATGTTGCGTCAGACGAGTGTTGATAGGGTTAAGTACAGCTCCAGCC
ATGGGAACGGCAAAGTGCATCTCATACATGGCCGGTGTGTTTGGAGCAACAACGGATACC
TAAACACATAATTATGGGTTAAGTACAGTCACAGATACTCTATACAAATGAATGTTTATC
TCAGATAAACGGACTATCTCTAAAAAAACTAAAACTGTTCAGAGAAAAAATAAGGTAAGG
ATAGTGTGACATACCACATCGTTCTTGCCGATATTAAGAGATATGAGAGAAGCGATGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
CGTCCAAATGG
   
Sequence Name 19-K033212-022-751-B10C-8409
GenBank Accession LN494659 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K033212-022-751-B10C-8409
CATTTACTCGCTTCTCTCATATCTCTTAATATCGGCAAGAACGATGTGGTATGTCACACT
ATCCTTACCTTATTTTTTCTCTGAACAGTTTTAGTTTTTTTAGAGATAGTCCGTTTATCT
GAGATAAACATTCATTTGTATAGAGTATCTGTGACTGTACTTAACCCATAATTATGTGTT
TAGGTATCCGTTGTTGCTCCAAACACACCGGCCATGTATGAGATGCACTTTGCCGTTCCC
ATGGCTGGAGCTGTACTTAACCCTATCAACACTCGTCTAGACGCAACATCCATTGCCGCA
ATCCTCCGCCACGCCAAGCCCAAGATTTTATTCATATACC

Last Updated on 06.12.2018 16:11