Sequences from confirmation amplicon


Confirmation sequences for line 757A06 and hit At3g55710 are shown.
Line ID 757A06
Locus At3g55710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20674684
Primers used for PCR 27qp - 8474, show primer details
   
Sequence Name 53-K115558-0022-757-A06C-27qp
GenBank Accession LN508252 [GenBank]
Sequencing primer gene specific primer, 27qp: 5'-CCTTCCACCATGTCGTTAAGTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K115558-0022-757-A06C-27qp
TTTTATACGGAAGATCCTTCACTTTCAAGGTGGAAGCTCTGTCACTAGCTCATCTAATCT
AGAACCTGAAGTTAAAAATAGTGGGTGGCAAATCTTTAATATTGCTAAAAAAATGTTAAG
AAACTTTTTTTTCAACCAGGAATCCTAAACCAAAACGGAAACTAATCCTTCGAGGCGAAA
ATTTTGGATTATATAATCCAATAACATGTATTATAACTACCCAATTGCAATTATTTTATA
TAAAAAACTGTTTTATAGAGAAGTGACAAGTGGTGTAATAAAGTAACCGTTATTATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 50-K115557-0022-757-A06C-8409
GenBank Accession LN507275 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K115557-0022-757-A06C-8409
ATTACATTGAATAATAACGGTTACTTTATTACACCACTTGTCACTTCTCTATAAAACAGT
TTTTTATATAAAATAATTGCAATTGGGTAGTTATAATACATGTTATTGGATTATATAATC
CAAAATTTTCGCCTCGAAGGATTAGTTTCCGTTTTGGTTTAGGATTCCTGGTTGAAAAAA
AAGTTTCTTAACATTTTTTTAGCAATATTAAAGATTTGCCACCCACTATTTTTAACTTCA
GGTTCTAGATTAGATGAGCTAGTGACAGAGCTTCCACCTTTGAAAGTGAAGGATCTTCCT
GTAATAAAAACGAAAGAGCCTGAGGGACTAAACCGAATACTTAACGACATGGGTGGAAGG

Last Updated on 06.12.2018 16:11