Sequences from confirmation amplicon


Confirmation sequences for line 758F05 and hit At5g03850 are shown.
Line ID 758F05
Locus At5g03850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1028585
Primers used for PCR CF52 - 8474, show primer details
   
Sequence Name 36-K106288-0022-758-F05C-8409
GenBank Accession LN502233 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K106288-0022-758-F05C-8409
GAGCCTTTACATTGAAGCCTGTAAGGATATCACCTTCCCTCACTGGTCCTTTAACATTCC
TCATGATGTAACGATCAGAGTCGGTAAACTTCACTCGAACCTGAGTAACCTGACCCCTTG
AACCAGTCCTACCCATGACCTTAACCACCACAGCGTGCTTGATCTGAGAATCCATTCTGA
TTCAATCAACCAAAACCATGAAAATTCACATTAAAACATCAATCAAATGCATACACAATC
AGTACACAGTTTCATGTAAAGCAATCAACTTTACAGAGATTATCACAATCAGAATTCCTT
GTTATGAAAGTTTCAATCTTCAGAGTTTCTCATCGTAACAGGAGCAACACTAGGGTTTCA
ATTATCTAAGAAAACAGGGGCTAACTTCA
   
Sequence Name 36-K106289-0022-758-F05C-CF52
GenBank Accession LN502234 [GenBank]
Sequencing primer gene specific primer, CF52: 5'-TTGAAGTTAGCCCCTGTTTTCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K106289-0022-758-F05C-CF52
GATGAGAAACTCTGAAGATTGAAACTTTCATAACAAGGAATTCTGATTGTGATAATCTCT
GTAAAGTTGATTGCTTTACATGAAACTGTGTACTGATTGTGTATGCATTTGATTGATGTT
TTAATGTGAATTTTCATGGTTTTGGTTGATTGAATCAGAATGGATTCTCAGATCAAGCAC
GCTGTGGTGGTTAAGGTCATGGGTAGGACTGGTTCAAGGGGTCAGGTTACTCAGGTTCGA
GTGAAGTTTACCGACTCTGATCGTTACATCATGAGGAATGTTAAAGGACCAGTGAGGGAA
GGTGATATCCTTACATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCACCGGTTATTAT
Primers used for PCR CF53 - 8474, show primer details
   
Sequence Name 69-K118446-0022-758-F05C-CF53
GenBank Accession LN512284 [GenBank]
Sequencing primer gene specific primer, CF53: 5'-CCAGGGTAGAAGAAAGTGATTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K118446-0022-758-F05C-CF53
CTTTATTTGAGCAGAGATTTCAACATTAGGGTAGAAACATATTTGCTTTCAAATTTTATC
TTAGCCTTGTAGCTTCCTATATCAGTTTTGGTTGATACATTTCAGAGACATTTTGGACTC
GATTAAAGCTTCGATTTTCAATTGGAAAAACCAAACCAATATAACCATTCTTCTACGAAT
CCAAGATCTGTGGCGATGGCAAGACCACACCACTGCTTACCCAAGTTATAAACATAACCC
AAAACACATGATTCAGGTAGAACAAGATGGAGAATAGAGCAAATTCTTGATGAACAATAT
AAAAAAGGGATGAAGAGATTTTGAATACCAAACAACTTATAAATCCAGCAGCAATTGTCT
TAAAGCAACAACAAAATTAAGACTCCAAATGCATAATCCACTAATCCAATCAAAGAAAGC
AAAAGAGAAATAACCAAATGTTTTCTTGTTTCCGCATTTTGGAGTATGAAGAAGAAGATC
AACGAAGTCCGGGAATATATCCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 29-K118445-0022-758-F05C-8409
GenBank Accession LN499484 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K118445-0022-758-F05C-8409
TCATTGAATATATCCTGGGATATATTCCCGGACTTCGTTGATCTTCTTCTTCATACTCCA
AAATGCGGAAACAAGAAAACATTTGGTTATTTCTCTTTTGCTTTCTTTGATTGGATTAGT
GGATTATGCATTTGGAGTCTTAATTTTGTTGTTGCTTTAAGACAATTGCTGCTGGATTTA
TAAGTTGTTTGGTATTCAAAATCTCTTCATCCCTTTTTTATATTGTTCATCAAGAATTTG
CTCTATTCTCCATCTTGTTCTACCTGAATCATGTGTTTTGGGTTATGTTTATAACTTGGG
TAAGCAGTGGTGTGGTCTTGCCATCGCCACAGATCTTGGATTCGTAGAAGAATGGTTATA
TTGGTTTGGTTTTTCCAATTGAAAATCGAAGCTTTAATCGAGTCCAAAATGTCTCTGAAA
TGTATCAACCAAAACTGATATAGGAAGCTACAAGGCTAAGATAAAATTTGAAAGCAAATA
TGTTTCTACCCTAATGTTTGAAATCTCTGCTTCAAATTAAAGTAGTACTCCAACCAGAGC
AGCTAATACCTCATCAGAATCACTTTCTCTTACCCTG
Primers used for PCR 25mg - 8474, show primer details
   
Sequence Name 70-K118446-0022-758-F05C-25mg
GenBank Accession LN512466 [GenBank]
Sequencing primer gene specific primer, 25mg: 5'-TTATTGAAAACCGAGTACCGAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K118446-0022-758-F05C-25mg
ATCCCTTATTCTTTCGTCTCTGTAAATTAGGGTTTTTGCCTCTCACGCTTATCGCCGCAT
CTCCGTCAACCTTCATCTACAGGTATCTTCATCGTTTACAAATCTTGTCTGTTGTATCTC
GAATCGATTGCTATCTCTTGTACATTTTCTTCTGCTAAATCTGTCTTGTATCGAATCTGT
TATTTGAAGTTAGCCCCTGTTTTCTTAGATAATTGAAACCCTAGTGTTGCTCCTGTTACG
ATGAGAAACTCTGAAGATTGAAACTTTCATAACAAGGAATTCTGATTGTGATAATCTCTG
TAAAGTTGATTGCTTTACATGAAACTGTGTACTGATTGTGTATGCATTTGATTGATGTTT
TAATGTGAATTTTCATGGTTTTGGTTGATTGAATCAGAATGGATTCTCAGATCAAGCACG
CTGTGGTGGTTAAGGTCATGGGTAGGACTGGTTCAAGGGGTCAGGTTACTCAGGTTCGAG
TGAAGTTTACCGACTCTGATCGTTACATCATGAGGAATGTTAAAGGACCAGTGAGGGAAG
GTGATATCCTTACATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCACAGGTTATT
   
Sequence Name 30-K118445-0022-758-F05C-oriL
GenBank Accession LN499918 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K118445-0022-758-F05C-oriL
GCCATTTACATTGAAGCCTGTAAGGATATCACCTTCCCTCACTGGTCCTTTAACATTCCT
CATGATGTAACGATCAGAGTCGGTAAACTTCACTCGAACCTGAGTAACCTGACCCCTTGA
ACCAGTCCTACCCATGACCTTAACCACCACAGCGTGCTTGATCTGAGAATCCATTCTGAT
TCAATCAACCAAAACCATGAAAATTCACATTAAAACATCAATCAAATGCATACACAATCA
GTACACAGTTTCATGTAAAGCAATCAACTTTACAGAGATTATCACAATCAGAATTCCTTG
TTATGAAAGTTTCAATCTTCAGAGTTTCTCATCGTAACAGGAGCAACACTAGGGTTTCAA
TTATCTAAGAAAACAGGGGCTAACTTCAAATAACAGATTCGATACAAGACAGATTTAGCA
GAAGAAAATGTACAAGAGATAGCAATCGATTCGAGATACAACAGACAAGATTTGTAAACG
ATGAAGATACCTGTAGATGAAGGTTGACGGAGATGCGGCGATAAGCGTGAGAGGCAAAAA
CCCTAATTTTACAGAGACGAAAGAATAAGGGATTTCTTTGCATTTTATAGGCCTGCTTAA
TTTCGGTACTCGTTTTTTCAAT

Last Updated on 06.12.2018 16:11