Sequences from confirmation amplicon


Confirmation sequences for line 759B02 and hit At1g75110 are shown.
Line ID 759B02
Locus At1g75110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28195041
Primers used for PCR 12kz - 8474, show primer details
   
Sequence Name 44-K113872-0022-759-B02C-12kz
GenBank Accession LN505255 [GenBank]
Sequencing primer gene specific primer, 12kz: 5'-CTTAGAGAGTGTATCTGCCACACG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K113872-0022-759-B02C-12kz
TAGAAAACCCTGAATTGAAACCCATATCCTCATTGTGTGTGCATACCTAGCCCATCCCAT
TGATGGTTCATCAAACACATCGTTGAAGCCATAAGCCGTGTTATTGTCGTGACCATCACT
CATTGACTCAACATCAGAGTCTCTGTGTAAATGACTAAAAGGATTCTGCAAGAAGACAAT
GTCCACATCCGATAGAAGAACACTGTAACCAAGCTGCAAGAACTCTCTTAAGACGCGGAA
CTTCAGTCCGGAAACAGCGTGATTACCTCCGCTTTTTCCAACCATGTCCACTGCTTTATC
TGGATCTCGCTTGTAAAACACAACCTCCTTCGATTCGCAGAAATCTACATGGGATTGAAA
TCTACATGGATTGGTAATGAAGCCATTGTGAATCCTCATTTGTTTGCAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATCTCCG
   
Sequence Name 18-K113871-0022-759-B02C-8409
GenBank Accession LN494388 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K113871-0022-759-B02C-8409
TCATTGAATATATCCTGCAACAAATGAGGATTCACAATGGCTTCATTACCAATCCATGTA
GATTTCAATCCCATGTAGATTTCTGCGAATCGAAGGAGGTTGTGTTTTACAAGCGAGATC
CAGATAAAGCAGTGGACATGGTTGGAAAAAGCGGAGGTAATCACGCTGTTTCCGGACTGA
AGTTCCGCGTCTTAAGAGAGTTCTTGCAGCTTGGTTACAGTGTTCTTCTATCGGATGTGG
ACATTGTCTTCTTGCAGAATCCTTTTAGTCATTTACACAGAGACTCTGATGTTGAGTCAA
TGAGTGATGGTCACGACAATAACACGGCTTATGGCTTCAACGATGTGTTTGATGAACCAT
CAATGGGATGGGCTAGGTATGCACACACAATGAGGATATGGGTTTTCAATTCAGGGTTTT
TCTATCTAAGACCAACTATTCCTTCAATAGACCTACTCGATCGTGTGGCAGATACACTCT
CTAA

Last Updated on 06.12.2018 16:11