Sequences from confirmation amplicon


Confirmation sequences for line 770C01 and hit At5g12370 are shown.
Line ID 770C01
Locus At5g12370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4005603
Primers used for PCR 15um - 8474, show primer details
   
Sequence Name 51-K117497-0022-770-C01C-15um
GenBank Accession LN507621 [GenBank]
Sequencing primer gene specific primer, 15um: 5'-TTTTGTTTTGTCATGCAAACTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K117497-0022-770-C01C-15um
ATTTGACAGAGTCCTTGTTTTCCTCGCACAAGGATGAATACCCTGAGCATGAGCGGGCCT
CCTTAAAACAACTATATCAAGCAAAGGTTTAATATGATTATTTCATTTTTCTCATTGAAG
CTAGTTGTTATGTTGTTCATTAATGTGAAGTGAATGAGTTTTTCTCTACAGATGGAAGAA
CTGCGTGCTGAGAGTCAGCAAGTCTCAGAGTCATCTGGGACAATAGGACGCTCTAAGGGT
GCTTCAATATCATCATCTCTGCAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCACGGATTATTAT
   
Sequence Name 19-K117496-0022-770-C01C-8409
GenBank Accession LN494938 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K117496-0022-770-C01C-8409
CATTTGCAGAGATGATGATATTGAAGCACCCTTAGAGCGTCCTATTGTCCCACATTACTC
TGAGACTTGCTGACTCTCAGCACGCAGTTCTTCCATCTGTAGAGAAAAACTCATTCACTT
CACATTAATGAACAACATAACAACTAGCTTCAATGAGAAAAATGAAATAATCATATTAAA
CCTTTGCTTGATATAGTTGTTTTAAGGAGGCCCGCTCATGCTCAGGGTATTCATCCTTGT
GCGAGGAAAACAAGGACTCTGTCAAATCTGCACAGTCAAAACAAAGAGTTGTCAGCACCA
ACGAAAGTTTGCATGACAAAACAAAA

Last Updated on 06.12.2018 16:11