Sequences from confirmation amplicon


Confirmation sequences for line 772A04 and hit At5g17920 are shown.
Line ID 772A04
Locus At5g17920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5938455
Primers used for PCR TK45 - 8474, show primer details
   
Sequence Name 47-K103676-0022-772-A04C-TK45
GenBank Accession LN506178 [GenBank]
Sequencing primer gene specific primer, TK45: 5'-GTCTCCTAAATTTAATCCTTGGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K103676-0022-772-A04C-TK45
TTTCTTATTGTTGTGATGCTATGGTTTCTTTATCTCTCTTTTACTAGGGTCTCAGAGGAG
GACTACGTTAAAGCCATCAAGGAAGAGATCAAGAAAGTTGTTGACCTCCAAGAGGAACTT
GACATCGATGTTCTTGTCCACGGAGAGCCAGAGGTGAATTTTTTTTATTATTCTATGTTT
TTGCCTGATATTTCTAGTAATCCTTGGTACTGTTTCTGATGAGACATGTTTTCACAATTT
TGTAGAGAAACGACATGGTTGAGTACTTTGGTGAGCAGTTGTCTGGTTTTGCCTTCACTG
CAAACGGATGGGTCCAATCTTATGGATCTCGCTGTGTGAAGCCACCAGTTATCTATGGTG
ATGTGAGCCGTCCCAAGGCAATGACCGTCTTCTGGTCCGCAATGGCTCAGAGCATGACCT
CTCGCCCAATGAAGGGTATGCTTACTGGTCCCGTCACCATTCTCAACTGGTCCTTTGTCA
GGAACGACCAGCCCAGGTACATAATGTTAATATGATTCCAGGTACATGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 63-K103677-0022-772-A04C-8409
GenBank Accession LN510971 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K103677-0022-772-A04C-8409
GCCTTTACATGTACCTGGAATCATATTAACATTATGTACCTGGGCTGGTCGTTCCTGACA
AAGGACCAGTTGAGAATGGTGACGGGACCAGTAAGCATACCCTTCATTGGGCGAGAGGTC
ATGCTCTGAGCCATTGCGGACCAGAAGACGGTCATTGCCTTGGGACGGCTCACATCACCA
TAGATAACTGGTGGCTTCACACAGCGAGATCCATAAGATTGGACCCATCCGTTTGCAGTG
AAGGCAAAACCAGACAACTGCTCACCAAAGTACTCAACCATGTCGTTTCTCTACAAAATT
GTGAAAACATGTCTCATCAGAAACAGTACCAAGGATTACTAGAAATATCAGGCAAAAACA
TAGAATAATAAAAAAAATTCACCTCTGGCTCTCCGTGGACAAGAACATCGATGTCAAGTT
CCTCTTGGAGGTCAACAACTTTCTTGATCTCTTCCTTGATGGCTTTAACGTAGTCCTCCT
CTGAGACCCTAGTAAAAGAGAGATTAAAGAAACCATTAGCATCACAACAATTTAAGAAAA
TGTGTGACGCANAAGCCCAAGGATTAAATTT

Last Updated on 06.12.2018 16:11