Sequences from confirmation amplicon


Confirmation sequences for line 783G01 and hit At2g40700 are shown.
Line ID 783G01
Locus At2g40700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16979269
Primers used for PCR 86zi - 8474, show primer details
   
Sequence Name 19-K129516-0022-783-G01C-86zi
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 86zi: 5'-AGTTGGTAGAACAGCTCGTATTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K129516-0022-783-G01C-86zi
ACCGATTGAATAGACTATCTTAAGGAGCTGAAGAACATGGTGCATCACTTACAGAGTACC
CTCTTATGAAAGTACTTGATAAATTTCCCATACCCGGCAATATGCCCCGAATCAAAAAGG
TCCTATCTCTAGAATCCCATCCATGGGTCATATCTCTGCAGAGAGCTCTTGAATCCCTTA
CTTACGCCGAGGTACGATTCTCTTCAATGTGTTCCTCCCTCTCTATGTGATTAATTTACA
GTCTGATGGTCTAAATGTCTCATTGTTTGTATCTGTTTTATTCACAGCCAAAGATGAAGA
GCCTGGCCAAGAATGCATTTGTCTCTTGGGTTAGGGGATATGCAGCTCACAAGGGAGAGC
TCAAGAGTATTTTCGTGGTGAAGAAGCTTCACTTGGGTCATGTCGCCAAGAGTTTTGCTC
TGAGAAATCTACATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 09-K129513-0022-783-G01C-oriL
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K129513-0022-783-G01C-oriL
CATTTCATTGAATATATCCATGTAGATTTCTCAGAGCAAAACTCTTGGCGACATGACCCA
AGTGAAGCTTCTTCACCACGAAAATACTCTTGAGCTCTCCCTTGTGAGCTGCATATCCCC
TAACCCAAGAGACAAATGCATTCTTGGCCAGGCTCTTCATCTTTGGCTGTGAATAAAACA
GATACAAACAATGAGACATTTAGACCATCAGACTGTAAATTAATCACATAGAGAGGGAGG
AACACATTGAAGAGAATCGTACCTCGGCGTAAGTAAGGGATTCAAGAGCTCTCTGCAGAG
ATATGACCCATGGATGGGATTCTAGAGATAGGACCTTTTTGATTCGGGGCATATTGCCGG
GTATGGGAAATTTATCAAGTACTTTCATAAGAGGGTACTCTGTAAGTGATGCACCATGTT
TCTTCAGCTCCTTAAGATAGTCTATTTCAATCGGTTGTAGAAATAACAAGGCTTCTCCTT
TTTCACCAATACGAGCTGTTCTACCAACT
Primers used for PCR 39zl - 8474, show primer details
   
Sequence Name 44-K129518-0022-783-G01C-39zl
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 39zl: 5'-TCATTGAAGTTTTGTCTTTTACGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K129518-0022-783-G01C-39zl
CATGTTTTGTATTCGAAAAGCAAAAAAAGGTCTGGTTTCTTTCTCTCTTATCTTTTGCCT
TCCAATTTTCAGTACTTGTTGTTTTTTGAATAGGTCTAATGAGTTCCTTTTAGGTTTGAC
TTAGACATACAACTTCAGTTATGCCAATTAAGGTGTAACGTTTGCTTAGGATTAAATAAA
TAACCCAACACATGAACACAAATAGTGAAAATATTCATACACATCTACTTAACACAAATG
CAACTAAACGCAACACAAAGTTCGATCACTCGATACTTTGCTTTATTTTTCTCAAAATTG
TTTTGGTTGGAGGTGATGTTGCTTTGCTAAGTTTCTTACAAATAAAAGAAAAATTTACAT
TTTGAAACGGCTTCTGGATTTTAAGCCGATCGCAATGGAGACATTGTTGTTGTGAGTAAG
CAATGGAACCATCTCAAGCAACTCTTTGATAGCAAATCTAGATTTTCATAGATTCTAAAC
CGAATTTTCATAAAAAAAACATTTAATAAAAACAGTGTTTTTGTGATCCATATTGATTTT
TTTTTAGAAATTTCCTGAAGAAAGTAAACATCTGGTAAGCAAATTTTGTGAATCTTTAGC
TTTCTGGCCAATACAAGTAATATTGTTAACAAACATGATAAAGCTCCCAACTTCTTTTCG
ATATTTCAAAATATTCGATGAGCAAACCAGCTCCTTAACTACAAAACTTACGCCACGGTT
TATGGTGGCGAAACCGATTTGATCAAGTTTTTTGTGTACTTCTATTGCCAGTACCACTCA
TCTTCTTTCTCTTCTTACCTTGTTGCCCCTTTTGACGTTCGTCTCTCTTTCTCTTCATTG
TTTCC
   
Sequence Name 23-K129517-0022-783-G01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K129517-0022-783-G01C-8409
ACAGTTGGGAATCCATCACAAGGAAACAATGAAGAGAAAGAGAGACGAACGTCAAAAAGG
GCAACAAGGTAAGAAGAGAAAGAAGATGAGTGGTACTGGCAATAGAAGTACACAAAAAAC
TTGATCAAATCGGTTTCGCCACCATAAACCGTGGCGTAAGTTTTGTAGTTAAGGAGCTGG
TTTGCTCATCGAATATTTTGAAATATCGAAAAGAAGTTGGGAGCTTTATCATGTTTGTTA
ACAATATTACTTGTATTGGCCAGAAAGCTAAAGATTCACAAAATTTGCTTACCAGATGTT
TACTTTCTTCAGGAAATTTCTAAAAAAAAATCAATATGGATCACAAAAACACTGTTTTTA
TTAAATGTTTTTTTTATGAAAATTCGGTTTAGAATCTATGAAAATCTAGATTTGCTATCA
AAGAGTTGCTTGAGATGGTTCCATTGCTTACTCACAACAACAATGTCTCCATTGCGATCG
GCTTAAAATCCAGAAGCCGTTTCAAAATGTAAATTTTTCTTTTATTTGTAAGAAACTTAG
CAAAGCAACATCACCTCCAACCAAAACAATTTTGAGAAAAATAAAGCAAAGTATCGAGTG
ATCGAACTTTGTGTTGCGTTTAGTTGCATTTGTGTTAAGTAGATGTGTATGAATATTTTC
ACTATTTGTGTTCATGTGTTGGGTTATTTATTTAATCCTAAGCAAACGTTACACCTTAAT
TGGCATAACTGAAGTTGTATGTCTAAGTCAAACCTAAAAGGAACTCATTAGACCTATTCA
AAAAACAACAAGTACTGAAAATTGGAAGGCAAAAGATAAGAGAGAAAGAAACCAGACCTT
TTTTTGCTTTTCTGAATCAAAACATGTTGTTGAT

Last Updated on 06.12.2018 16:11