Sequences from confirmation amplicon


Confirmation sequences for line 786F05 and hit At1g01040 are shown.
Line ID 786F05
Locus At1g01040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29930
Primers used for PCR HP60 - 8474, show primer details
   
Sequence Name 67-K033720-022-786-F05C-8409
GenBank Accession LN511747 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K033720-022-786-F05C-8409
TCGGACTGAGCCTTTACATTGAATATATCCTGTGAAAAATAGATGTCTTGTGATGAGATG
ATCCAAGACCGCGTCACCAACAAATTCCAATCTCTGGTAACACGAAACACCTGAAGATGG
TCTTGAAGCATGTGTTATAGCTTCAACAAGAAGACCTTTCTCTTTAAACTCATATTTAAG
AGCTCTCTCAAGACCAACAAAGTCGATGCTCTTGAGCACACTCTCTGGAACATTAACATT
TTTCAATGTTCCATCGACTTCATCAGGATCATCCTCCACGTGAATCCCAATCCATTTCAT
CAAATGATTAGCTGCAATCTTACCCCCTTCGACGTAATAAACACCAATCAAAGCCTCAAC
AACATCAGCTAACGTTTTGCTAGATAAAACTCGGTACGAACTCAAATCACCCTCTAGTTC
ACCATCCTCCATCTC
   
Sequence Name 29-K033720-022-786-F05C-HP60
GenBank Accession LN499189 [GenBank]
Sequencing primer gene specific primer, HP60: 5'-GAGATGGAGGATGGTGAACTAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K033720-022-786-F05C-HP60
CAAACGTTAGCTGATGTTGTTGAGGCTTTGATTGGTGTTTATTACGTCGAAGGGGGTAAG
ATTGCAGCTAATCATTTGATGAAATGGATTGGGATTCACGTGGAGGATGATCCTGATGAA
GTCGATGGAACATTGAAAAATGTTAATGTTCCAGAGAGTGTGCTCAAGAGCATCGACTTT
GTTGGTCTTGAGAGAGCTCTTAAATATGAGTTTAAAGAGAAAGGTCTTCTTGTTGAAGCT
ATAACACATGCTTCAAGACCATCTTCAGGTGTTTCGTGTTACCAGAGATTGGAATTTGTT
GGTGACGCGGTCTTGGATCATCTCATCACAAGACATCTATTTTTCACAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
CATTCACGTCCAAATGGG

Last Updated on 06.12.2018 16:11