Sequences from confirmation amplicon


Confirmation sequences for line 787F05 and hit At2g17310 are shown.
Line ID 787F05
Locus At2g17310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7531226
Primers used for PCR 36dl - 8474, show primer details
   
Sequence Name 42-K112758-0022-787-F05C-36dl
GenBank Accession LN504520 [GenBank]
Sequencing primer gene specific primer, 36dl: 5'-GTGATATTCCCCAAATTTTTCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K112758-0022-787-F05C-36dl
CTTTCAAAATAAGTTACACACATCAGTCTAGCTTCTCATTAAAAAGTGTTGCACTTAATT
GTTCTTGATTTGTTATATAAACTTCAAAAATATTATTGGCTCAATAATTTAGAAAGAACT
TTTCAATCTTTCACTTTGTTAACATGTCTAAGTCACATGTTAATTACATATATGTCTATA
TTAAATGTCAGAATCTAGCTTTAATTTGCAAAGTTACTTTCCTTTGCTAACAGAGGATAA
ATACAGTCTCTTCTCCTATTTTTTTTTGTTCAGAGCTATAACTCCAAAGTCCAAACTAAA
CTAAGAACAGAAAACCTCATGGCTCTTCCGTGGTATAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 02-K112757-0022-787-F05C-8409
GenBank Accession LN487106 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K112757-0022-787-F05C-8409
ATTTCATTGAATATATCCTATACCACGGAAGAGCCATGAGGTTTTCTGTTCTTAGTTTAG
TTTGGACTTTGGAGTTATAGCTCTGAACAAAAAAAAATAGGAGAAGAGACTGTATTTATC
CTCTGTTAGCAAAGGAAAGTAACTTTGCAAATTAAAGCTAGATTCTGACATTTAATATAG
ACATATATGTAATTAACATGTGACTTAGACATGTTAACAAAGTGAAAGATTGAAAAGTTC
TTTCTAAATTATTGAGCCAATAATATTTTTGAAGTTTATATAACAAATCAAGAACAATTA
AGTGCAACACTTTTTAATGAGAAGCTAGACTGATGTGTGTAACTTTATTTTTGAAAGCAA
CAAAGCTGAAAATAAAATTGTAACGGTAGGAGAAGGAAAAATTTGGGNAATATC

Last Updated on 06.12.2018 16:11