Sequences from confirmation amplicon


Confirmation sequences for line 794C09 and hit At3g50920 are shown.
Line ID 794C09
Locus At3g50920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18922603
Primers used for PCR 17um - 8474, show primer details
   
Sequence Name 26-K117753-0022-794-C09C-17um
GenBank Accession LN498112 [GenBank]
Sequencing primer gene specific primer, 17um: 5'-AATCTTGATTTTAACAAACGGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K117753-0022-794-C09C-17um
AATTCGAGGGAAACAATTTTAAGCTTTATAAGTAAAGCTTGGTATTGCTTATCTCTCGTC
TTTGAACCAGTTAAGTACAACGTACGCTGCGAAAGCTAGCGCAAATGTAGCTGCAAACAG
AAATACAGATATTTGCACTAATAGAGATGCCTCGAAGGCTTCACGAAGAAGCGAATTCCA
CATTGTGTACCATAAGATGCAGAAAAGAGAACCAACAATGGCACCCACCACCACTTGACT
GCTTGTGTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCG
   
Sequence Name 10-K117752-0022-794-C09C-8409
GenBank Accession LN490847 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117752-0022-794-C09C-8409
CACACAAGCAGTTCAAGAGGTGGTGGGTGCCATTGTTGGTTCTCTTTTCTGCATCTTATG
GTACACAATGTGGAATTCGCTTCTTCGTGAAGCCTTCGAGGCATCTCTATTAGTGCAAAT
ATCTGTATTTCTGTTTGCAGCTACATTTGCGCTAGCTTTCGCAGCGTACGTTGTACTTAA
CTGGTTCAAAGACGAGAGATAAGCAATACCAAGCTTTACTTATAAAGCTTAAAATTGTTA
TCCCTCAAATTCTTTTCAAAATATCGAAATGAAGGATTCAGAGCCCGTTTGTTAAAATCA
AGATTA

Last Updated on 06.12.2018 16:11