Sequences from confirmation amplicon


Confirmation sequences for line 795E06 and hit At3g13170 are shown.
Line ID 795E06
Locus At3g13170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4232855
Primers used for PCR TA21 - 8474, show primer details
   
Sequence Name 36-K030142-022-795-E06C-8409
GenBank Accession LN502100 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K030142-022-795-E06C-8409
AATTGAAATGGATATGCCTGATATCATTTTAAGTGATGCTTTATGCTCTTTAGAGAAGTC
AAATCCTTATCTCAGCTGATATCAGGAAGTATAAGATTCACTATCTATGGTGGCATCAGG
AACTATAATTGGATGTCATTTTCCTGCTTGCAGATATTGTTAGCTTAGCTGAGTACATAC
TGGTTGTGGAAAAAGAAACAGGTAAGAAAGACCTCTTATTTATGTAGTAACTAGGAAGAT
GGTTTTCTTTCTTCATGACTAAATCGCTTTCATCCATGGTTTATAGTATTCCAGCGTTTA
GCAAATGACATGTTTTGCAAGACGAACCGCTGCATTGTTATCACAGTAAGAAATCTGCTT
CATTCTTACTGATTTCAGTTTTGACTAGTTTTCATTTCCATTTTATGTTTGACATCAAAG
TTACGATGTTTT
   
Sequence Name 36-K030142-022-795-E06C-TA21
GenBank Accession LN502101 [GenBank]
Sequencing primer gene specific primer, TA21: 5'-GCCAAAAACAATCGTAACTTTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K030142-022-795-E06C-TA21
AACTAGTCAAACTGAAATCAGTAAGAATGAAGCAGATTTCTTACTGTGATAACAATGCAG
CGGTTCGTCTTGCAAAACATGTCATTTGCTAAACGCTGGAATACTATAAACCATGGATGA
AAGCGATTTAGTCATGAAGAAAGAAAACCATCTTCCTAGTTACTACATAAATAAGAGGTC
TTTCTTACCTGTTTCTTTTTCCACAACCAGTATGTACTCAGCTAAGCTAACAATATCTGC
AAGCAGGAAAATGACATCCAATTATAGTTCCTGATGCCACCATAGATAGTGAATCTTATA
CTTCCTGATATCAGCTGAGATAAGGATTTGACTTCTCTAAAGAGCATAAAGCATCACTTA
AAATGATATCAGGCATATCCATTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11