Sequences from confirmation amplicon


Confirmation sequences for line 796F03 and hit At1g53210 are shown.
Line ID 796F03
Locus At1g53210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19847091
Primers used for PCR NH72 - 8474, show primer details
   
Sequence Name 62-K102594-0022-796-F03C-8409
GenBank Accession LN510727 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K102594-0022-796-F03C-8409
TCGGAATGAGCCTTTACAATTGAATATATCCTGCAAATGGCTTCAATGAAGAAAGACGGA
ATCCCTGTGGCTGCGGAGAAGTTGTTAACAGTGTCGACTAAAGGATCAGCAAATGCAGCT
GCAATGGCAGCTCCTAACAGTAGCAGCAGAGCTGCTTTAATGGTGATCCATTTTGGGTCT
GCAACCTCACCGCCTTCCTCATCATTCTCACCATTCTCGTTATCTCCCAACAAAGCATGC
TCTCTCTTTGTTTGCTGCAAAACAAGAAAAAGGATAAAACTAATTGCGTTAGATTCCAGA
TAAGTTTTGTGACCCAAGTCTGTTCAAATAAGGTTTCAACAGTCTTTCTAACCACATGGA
AATTGTCAAGGAATTTCATTGTTCGAGGGCCAGCTTCAGGACCAGATGGAGCACCTCCCA
TTGCCTGGATAAGCCATTGCTTAATTCCACGGACAAACTCTTCTTGATCAACTTGCTCAT
CAAGAGTCTTATCAAAATCTTGGAGAACTTTTCCCACAGCATCATCCTT
   
Sequence Name 62-K102595-0022-796-F03C-NH72
GenBank Accession LN510728 [GenBank]
Sequencing primer gene specific primer, NH72: 5'-TAGATTTTGACAAGGATGATGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K102595-0022-796-F03C-NH72
AGATTTGATAGACTCTTGATGAGCAAGTTGATCAAGAAGAGTTTGTCCGTGGAATTAAGC
AATGGCTTATCCAGGCAATGGGAGGTGCTCCATCTGGTCCTGAAGCTGGCCCTCGAACAA
TGAAATTCCTTGACAATTTCCATGTGGTTAGAAAGACTGTTGAAACCTTATTTGAACAGA
CTTGGGTCACAAAACTTATCTGGAATCTAACGCAATTAGTTTTATCCTTTTTCTTGTTTT
GCAGCAAACAAAGAGAGAGCATGCTTTGTTGGGAGATAACGAGAATGGTGAGAATGATGA
GGAAGGCGGTGAGGTTGCAGACCCAAAATGGATCACCATTAAAGCAGCTCTGCTGCTACT
GTTAGGAGCTGCCATTGCAGCTGCATTTGCTGATCCTTTAGTCGACACTGTTAACAACTT
CTCCGCAGCCACAGGGATTCCGTCTTTCTTCATTGAAGCCATTTGCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
ATTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11