Sequences from confirmation amplicon


Confirmation sequences for line 800C12 and hit At2g41470 are shown.
Line ID 800C12
Locus At2g41470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17293387
Primers used for PCR KD64 - 8474, show primer details
   
Sequence Name 13-K103735-0022-800-C12C-KD64
GenBank Accession LN492008 [GenBank]
Sequencing primer gene specific primer, KD64: 5'-TATAGGGTTCATTGAAGAATTGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K103735-0022-800-C12C-KD64
GCACTACTGCGAGTTCTCCAGCAACAGGTAGGGTTTAGCTGTTGAATTAGTCTCTGGTAA
TTGGAATATGGAAACCATCCCCAATCTGTATCTTTAGATTGTTGCTTTAGCTAGCTAGTT
ACTGGGGCTCCAACATGATTATGATTAGAAAAAGGTTGATCAATCTCATTTAGCCAAATA
GTATCTCTTTGAGCTAATAAAAGCCTTGACTTTTTTTCCAGATATGGATGTGGCTCCTCT
GAACAAGACAATGTGGTATATGCAATTATTTCCTTGTGTCCTCTACTGAATTTCCAAATC
ATATATATATATATATACACATATATGTCTTCTTCTTGCAGCTGCGGGAAAAGGTGGAAA
ATGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGC
   
Sequence Name 29-K103736-0022-800-C12C-8409
GenBank Accession LN499254 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K103736-0022-800-C12C-8409
GCCATTTACAATTGAATATATCCTGATCATTTTCCACCTTTTCCCGCAGCTGCAAGAAGA
AGACATATATGTGTATATATATATATATATGATTTGGAAATTCAGTAGAGGACACAAGGA
AATAATTGCATATACCACATTGTCTTGTTCAGAGGAGCCACATCCATATCTGGAAAAAAA
GTCAAGGCTTTTATTAGCTCAAAGAGATACTATTTGGCTAAATGAGATTGATCAACCTTT
TTCTAATCATAATCATGTTGGAGCCCCAGTAACTAGCTAGCTAAAGCAACAATCTAAAGA
TACAGATTGGGGATGGTTTCCATATTCCAATTACCAGAGACTAATTCAACAGCTAAACCC
TACCTGTTGCTGGAGAACTCGCAGTAGTTGCACATCTTCTTCCTCAAATCTGTTCGCCAA
TTCTTCAATGAACCCTA

Last Updated on 06.12.2018 16:11