Sequences from confirmation amplicon


Confirmation sequences for line 800C12 and hit At5g66880 are shown.
Line ID 800C12
Locus At5g66880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26710527
Primers used for PCR DM04 - 8474, show primer details
   
Sequence Name 85-K101748-0022-800-C12C-8409
GenBank Accession LN514525 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K101748-0022-800-C12C-8409
GCCATTTAAATTGATCCATTGCCTTTTTTCTTCCTCTCTTTCCTTATTCGTTCTCTTCTT
GAAGATATTTTTCCATTTATTTTCTCGAATCTTCTTCTTCTTCCTAGATTCCAGCGACTT
AACAACAACAACAACAACATATTCTCTGCTGGGTATTAGATTCGAATTTCTCTTTTTGTG
ATCAGAAATGGATCGAGCTCCGGTGACCACAGGACCGTTGGATATGCCGATTATGCACGA
CAGTGATCGATATGACTTCGTTAAGGATATTGGTTCTGGTAATTTCGGTGTTGCTCGTCT
TATGAGAGATAAACTCACTAAAGAGCTTGTTGCTGTCAAGTACATCGAGAGAGGAGACAA
GGTTTCATTTTTAAAAATCTCATCTTTTTCTATTTTTGGAAGTTGATGATATTTATTTAG
TCCAGTGATCGTTCATTTTTAGTGTTTGGTTTAGCTTTCATNTTCCAG
   
Sequence Name 85-K101754-0022-800-C12C-DM04
GenBank Accession LN514526 [GenBank]
Sequencing primer gene specific primer, DM04: 5'-ATCTGGAAAGTGAAAGCTAAACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K101754-0022-800-C12C-DM04
TATCATCAACTTCCAAAATAGAAAAAGATGAGATTTTTAAAAATGAAACCTTGTCTCCTC
TCTCGATGTACTTGACAGCAACAAGCTCTTTAGTGAGTTTATCTCTCATAAGACGAGCAA
CACCGAAATTACCAGAACCAATATCCTTAACGAAGTCATATCGATCACTGTCGTGCATAA
TCGGCATATCCAACGGTCCTGTGGTCACCGGAGCTCGATCCATTTCTGATCACAAAAAGA
GAAATTCGAATCTAATACCCAGCAGAGAATATGTTGTTGTTGTTGTTGTTAAGTCGCTGG
AATCTAGGAAGAAGAAGAAGATTCGAGAAAATAAATGGAAAAATATCTTCAAGAAGAGAA
CGAATAAGGAAAGAGAGGAAGAAAAAAGGCAATGGATCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11