Sequences from confirmation amplicon


Confirmation sequences for line 814C02 and hit At2g21340 are shown.
Line ID 814C02
Locus At2g21340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9135145
Primers used for PCR 24nt - 8474, show primer details
   
Sequence Name 55-K114716-0022-814-C02C-24nt
GenBank Accession LN508889 [GenBank]
Sequencing primer gene specific primer, 24nt: 5'-TACCAGTAAGGTGCCTTCAAGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K114716-0022-814-C02C-24nt
GCAGAAATACGGTATTATGACCTTGTGCATCTGTTAGAATATGAAACGAATAACTGTTTA
AAAACTCTTCATTCTTCAAAATTTGTTGTCGGGAACATATGAGAAAAACTGTAAAGATAT
ACCTCGGATGTGACAACCTTGTCACGTGTGAAGATGCCAGGGAACAGCCATGGAACTGCT
GTGCCAATGGTTCCGACTACTATTCCTAGCGTAGCTCCGATGATAACTAGTGACTTCAGA
AGCACCCTAGCCTACAGACAACAGTACAAGCAGAGTTGCTTTAGGCAACAGTAAAACAAA
AAAATAAAACTGATGTGCAAATAAATGGTTTCAACTGACAACTTATGACCTACTTTAGGC
AAATTACGATTGATTCCGAATAAAAGCTCAGGCATAAAGGACTGTGCAGTTTGAGAGAGA
GGCTCCCCCCAAACCGTACTCATGGTATATATCTGAAGCATAACCTAGAGACCAAAAGAG
CCAAAGAAGTTATAGAGAAAACTCAAATCAGAAACATGGTTGGTGGTGAGATATTAAAAC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCA
   
Sequence Name 07-K114715-0022-814-C02C-8409
GenBank Accession LN489384 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K114715-0022-814-C02C-8409
CCTGTTTTATATCTCACCACCAACCATGTTTCTGATTTGAGTTTTCTCTATAACTTCTTT
GGCTCTTTTGGTCTCTAGGTTATGCTTCAGATATATACCATGAGTACGGTTTGGGGGGAG
CCTCTCTCTCAAACTGCACAGTCCTTTATGCCTGAGCTTTTATTCGGAATCAATCGTAAT
TTGCCTAAAGTAGGTCATAAGTTGTCAGTTGAAACCATTTATTTGCACATCAGTTTTATT
TTTTTGTTTTACTGTTGCCTAAAGCAACTCTGCTTGTACTGTTGTCTGTAGGCTAGGGTG
CTTCTGAAGTCACTAGTTATCATCGGAGCTACGCTAGGAATAGTAGTCGGAACCATTGGC
ACAGCAGTTCCATGGCTGTTCCCTGGCATCTTCACACGTGACAAGGTTGTCACATCCGAG
GTATATCTTTACAGTTTTTCTCATATGTTCCCGACAACAAATTTTGAAGAATGAAGAGTT
TTTAAACAGTTATTCGTTTCATATTCTAACAGATGCACAAGGTCATAATACCGTATTTTC
TTGCTTTATCCATCACTCCAAGTACTCACAGTCTTGAAGGCACNTAACTGGT

Last Updated on 06.12.2018 16:11