Sequences from confirmation amplicon


Confirmation sequences for line 814E05 and hit At2g40700 are shown.
Line ID 814E05
Locus At2g40700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16978542
Primers used for PCR 20zi - 8474, show primer details
   
Sequence Name 38-K126648-0022-814-E05C-20zi
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 20zi: 5'-CGGTACTGGAAAGACTATAGCGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K126648-0022-814-E05C-20zi
TCTCCAAGGTTGATCGTTCTCATGGAACTTTTGGTATGTGCTTTTGTTCTAGCTACAATT
TAGTATTTCTTTGCTGGTAAAGTGATGATTTTGATACTTGGCACTTGTTTGTGGCAGCTT
TGGTGATTGTGCCTACACGGGAACTATGCCTTCAGGTGTATGAAACCTTGGAGAAGTTAC
TGCATCGGTTCCATTGGATAGTCCCTGGCTATGTTATGGGAGGAGAGAAAAAGGCTAAAG
AAAAGGCTAGATTAAGGAAAGGTACAAATTTGTGAGAAGCGAGATGGTCACTTTTGAATC
CAGACTAATCGTTTTATTTCAGGAATATCCATTCTAATTGCAACCCCGGGACGCCTTCTT
GACCATTTGAAGAACACAGCTTCATTTGTGCACAAAAATCTGCGTTGGGTCATCTTTGAT
GAAGCTGATAGGTATAAATGCTGTACCCTGAAAGTTAATATCTTCCTTGATCCTGTGCTT
CCTTTAATTAATTCTTCCTTTGCATTTTGGCAGCATTCTCGAATTAGGTTATGGGAAGGA
GATAGAACAGATAATTAAGCTTCTAGGTTCAGGGCAAAACGAGCAAGGGGAAGAAGATGA
TATTGTTCCAAAGGGTATTCAGAAGCAGAACTTGTTATTGTCAGCAACATTGAATGATAA
AGTTAACGATCTTGCAAAACTTAGTTTGGACGATCCGGTAATGATTGGTCTTGACAACAC
CAAATTGCAACAAAATCTATCAATAGAGTCTCCTGCGGCTCCTGACTCTGATGCGGAAGA
TATGGTAATTCATGTTAACAAATCCGCAAACCCTTTATCTGAGGACTATGGAATACCATC
ACAGCTGGTACAGAGATATCTTAGAGGTACATTTATCTTCACTTGAATCTCTTTGTATTG
TTCTGTGCTTCTAGTTTGATACAAATATATATTACATGCAGTGCCATGTGTTGCACGGCT
TGTGGCACTTCTTTCTGTTTCTCAGAACTTTTTGAAAGAGAGCTTCTCAA
   
Sequence Name 31-K126647-0022-814-E05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K126647-0022-814-E05C-8409
CCAACGCGATCTTCTTCCTCCTGCTCCTACTTCCATGTAACCGGAACGTTTTGCACTTGA
GAAACAACTCTTTAGTCCCCTCCTCTTCTGTCTCAGAATTCGGTGGCCACTGGAATTCAC
TCAGAAGTGAGTAATGAAAATCTACAGCATCACGTGTCGAAAAGAAGACGACCACCTGTA
AGAAAACAGCCCCACAAAACCTCAATTAACGGAGAATTTTAAAATAAACAGACAGTTATA
TGACAGGAACTTCTGGCTTGAGATTTACCTTTTGAGAAGCTTCTCTTTCAAAAAGGTTCT
TGAGAACAGAAAGAAGTGCCACAAGCCGTGCACCACATGGCACTGCATGTAATATATATT
TGTATCAAACTAGAAGCACAGAACAATACAAAGAGATTCAAGTGAAGATAAATGTACCTC
TAAGATATCTCTGTACCAGCTGTGATGGTATTCCATAGTCCTCAGATAAAGGGTTTGCGG
ATTTGTTAACATGAATTACCATATCTTCCGCATCAGAGTCAGGAGCCGCAGGAGACTCTA
TTGATAGATTTTGTTGCAATTTGGTGTTGTCAAGACCAATCATTACCGGATCGTCCAAAC
TAAGTTTTGCAAGATCGTTAACTTTATCATTCAATGTTGCTGACAATAACAAGTTCTGCT
TCTGAATACCCTTTGGAACAATATCATCTTCTTCCCCTTGCTCGTTTTGCCCTGAACCTA
GAAGCTTAATTATCTGTTCTATCTCCTTCCCATAACCTAATTCGAGAATGCTGCCAAAAT
GCAAAGGAAGAATTAATTAAAGGAAGCACAGGATCAAGGAAGATATTAACTTTCAGGGTA
CAGCATTTATACCTATCAGCTTCATCAAAGATGACCCAACGCAGATTTTTGTGCACAAAT
GAAGCTGTGTTCTTCAAATGGTCAAGAAAGGCGTCCGGGGGTGCATTAGAATGGATATTC
CCTGAATAAACGATTAGTCTGGATCAAAAGTGACCATCTCGCTTCTCACAATTT

Last Updated on 06.12.2018 16:11