Sequences from confirmation amplicon


Confirmation sequences for line 818D11 and hit At2g02780 are shown.
Line ID 818D11
Locus At2g02780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:782184
Primers used for PCR EU76 - 8474, show primer details
   
Sequence Name 16-K103785-0022-818-D11C-EU76
GenBank Accession LN493403 [GenBank]
Sequencing primer gene specific primer, EU76: 5'-ATTTCTAGGTTGGAGAAGAGAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K103785-0022-818-D11C-EU76
GATCAATGTGTTCTCTTCTATCAAATTTTGAAACTTGTTAATGAATAATGTGCAGTAATG
AAGATAGAGAGAAAGAAGATGTGTACCAGTTTGGAGTGATACTACTACAAATCATCACAG
GGAAAGTAGTTGCTGCAGGATCTTCAGAGATGGGAAGTTTGAAGCTTCAGGTCTGTTCTG
TTTCAGTCAGAACCTTTTCATCTTTAAGAATCCGACACGGCTTACAAATTTAGATGTCCG
GCAGCGGCGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATG
   
Sequence Name 32-K103786-0022-818-D11C-8409
GenBank Accession LN500554 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K103786-0022-818-D11C-8409
ATTTACATTGAATATATCCTGCCGCCGCTGCCGGACATCTAAATTTGTAAGCCGTGTCGG
ATTCTTAAAGATGAAAAGGTTCTGACTGAAACAGAACAGACCTGAAGCTTCAAACTTCCC
ATCTCTGAAGATCCTGCAGCAACTACTTTCCCTGTGATGATTTGTAGTAGTATCACTCCA
AACTGGTACACATCTTCTTTCTCTCTATCTTCATTACTGCACATTATTCATTAACAAGTT
TCAAAATTTGATAGAAGAGAACACATTTGATCATATCTTAAGAAACAACAACAACCTCCG
AGGTTTTTTGGCTTGAGGCCTCTCTTCTCCAAACTAGAA
Primers used for PCR BB12 - 8474, show primer details
   
Sequence Name 57-K118446-0022-818-D11C-BB12
GenBank Accession LN509547 [GenBank]
Sequencing primer gene specific primer, BB12: 5'-TGTTTTTCAACTCCTTTTGCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K118446-0022-818-D11C-BB12
AGATATTTCGGTAGTCCATGAAACTCATCTTGACTTGTTTGAAACTTATTATTGTTTCTA
CAGTTTTTGCCTCTCTTTATGATCATTTCATTCATATATTGCTTTCATGGATGATTCATG
ATTCCCACTGCTTGGTCTCCATCCTTGCTGCACTTGAATTGTATACTGTAGATTCCACAC
AACGTCTTCTATGGAAGGACGCTTGCTCTGATCTTCGCAAAGACAGTTGATAGCAAACTC
TACTGTCGTCCTCAACGACTCATAAGCATATGATCCTTTAACAGATGGATCTGCTAAGCT
GCTCAATACTGATGGTTCATCTCTCAAACCGTTCTCCAGCGGCGGCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
CGGTTTATT
   
Sequence Name 17-K118445-0022-818-D11C-8409
GenBank Accession LN494063 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118445-0022-818-D11C-8409
GCCTTTCATTGAATATATCCTGCCGCCGCTGGAGAACGGTTTGAGAGATGAACCATCAGT
ATTGAGCAGCTTAGCAGATCCATCTGTTAAAGGATCATATGCTTATGAGTCGTTGAGGAC
GACAGTAGAGTTTGCTATCAACTGTCTTTGCGAAGATCAGAGCAAGCGTCCTTCCATAGA
AGACGTTGTGTGGAATCTACAGTATACAATTCAAGTGCAGCAAGGATGGAGACCAAGCAG
TGGGAATCATGAATCATCCATGAAAGCAATATATGAATGAAATGATCATAAAGAGAGGCA
AAAACTGTAGAAACAATAATAAGTTTCAAACAAGTCAAGATGTAGTTTCATGGACTACCT
GAAATATCTTTTATTAGCTAACTTAGTAATCTGTGAATAAAAAGCAAAAGGATTGGAAAA
AC

Last Updated on 06.12.2018 16:11