Sequences from confirmation amplicon


Confirmation sequences for line 830A02 and hit At3g04840 are shown.
Line ID 830A02
Locus At3g04840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1330418
Primers used for PCR 80ra - 8474, show primer details
   
Sequence Name 55-K116162-0022-830-A02C-80ra
GenBank Accession LN508910 [GenBank]
Sequencing primer gene specific primer, 80ra: 5'-TCCTCAAATTACTTCGCTGCTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K116162-0022-830-A02C-80ra
GGTTCTTGATTGATTGTTACAGTTGTGTATCTTGTGTTATAATGTTACTATTCATTTTCT
TATACATGTTGAGTGTAAAATGTTGTATTGGTGCCATGTACGGACTTTGAGACTGATGAT
GATGTCTGTTCTTACTCTTCCAGATTGCCTCAGAAGGCTTGAAACACAGAGTTTTTGATC
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCGCAGTTATTAT
   
Sequence Name 26-K116161-0022-830-A02C-8409
GenBank Accession LN498076 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K116161-0022-830-A02C-8409
ATTGAGATCAAAAACTCTGTGTTTCAAGCCTTCTGAGGCAATCTGGAAGAGTAAGAACAG
ACATCATCATCAGTCTCAAAGTCCGTACATGGCACCAATACAACATTTTACACTCAACAT
GTATAAGAAAATGAATAGTAACATTATAACACAAGATACACAACTGTAACAATCAATCAA
GAACCATAAGAATCAGACAAATCAGCTTAATCCTACGAAGAGTATAAGCAGCGAAGTAAT
TTGAGGA

Last Updated on 06.12.2018 16:11