Sequences from confirmation amplicon


Confirmation sequences for line 835F07 and hit At1g49830 are shown.
Line ID 835F07
Locus At1g49830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18445822
Primers used for PCR 15gg - 8474, show primer details
   
Sequence Name 28-K111953-0022-835-F07C-15gg
GenBank Accession LN498908 [GenBank]
Sequencing primer gene specific primer, 15gg: 5'-CAAGACATAAACCATTGCTTCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K111953-0022-835-F07C-15gg
TTCTCCGGTGTCTTCGTCGTCCTGACGACACATCAACTATTCATCAGTCACAACACCGTC
AAAATAGTAATCTTTTAAGTCTTAAAACAGTCGTTTCGAATCATGGAAAAATAAATATCT
TCCGGTTTTATTGATAAAGACATAGAGTGTAACAAAAAAAGAGGATACCAAGAAAAAAGT
GTAACAAAAAAATGAAATAAGATAATACAAAAGGATGATGGATGACTTACAAGAGGGTTA
TGTTTGAAGTAAGGGGTTGAAAGAACTTGAACTTGGTCTTGTAAGAATTTTATGTAACCC
ATTGTTTCATGCAACACTGATGCTGCATCTGTCTATTCAAATTTTCCAAATTACATTTTT
CTTAGTTAAAATGTAGAAAGTAAACTAATTTTAATTACCAAAACACATAATGTACCTCCA
AAACAGTAAAATATAGTCCGTGAATATCTCTCTACAAGTAAAGGTTTCAAGCTAATACCA
AATGACGTAACTATAGTAAAAGTTTCAAGTTTACATCAAATTTTCCTCTATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 12-K111952-0022-835-F07C-8409
GenBank Accession LN491640 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K111952-0022-835-F07C-8409
TCATTGAATATATAGAGGAAAATTTGATGTAAACTTGAAACTTTTACTATAGTTACGTCA
TTTGGTATTAGCTTGAAACCTTTACTTGTAGAGAGATATTCACGGACTATATTTTACTGT
TTTGGAGGTACATTATGTGTTTTGGTAATTAAAATTAGTTTACTTTCTACATTTTAACTA
AGAAAAATGTAATTTGGAAAATTTGAATAGACAGATGCAGCATCAGTGTTGCATGAAACA
ATGGGTTACATAAAATTCTTACAAGACCAAGTTCAAGTTCTTTCAACCCCTTACTTCAAA
CATAACCCTCTTGTAAGTCATCCATCATCCTTTTGTATTATCTTATTTCATTTTTTTGTT
ACACTTTTTTCTTGGTATCCTCTTTTTTTGTTACACTCTATGTCTTTATCAATAAAACCG
GAAGATATTTATTTTTCCATGATTCGAAACGACTGTTTTAAGACTTAAAAGATTACTATT
TTGACGGTGTTGTGACTGATGAATAGTTGATGTGTCGTCAGGACGACGAAGACACCGGAG
AAGTAAACCCGACGATGAANGTGAAAGAGTTGAGAAGCAATGGTTATTGTCTTG

Last Updated on 06.12.2018 16:11