Sequences from confirmation amplicon


Confirmation sequences for line 837H11 and hit At1g15130 are shown.
Line ID 837H11
Locus At1g15130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5207226
Primers used for PCR 02ae - 8474, show primer details
   
Sequence Name 76-K106292-0022-837-H11C-8409
GenBank Accession LN513405 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K106292-0022-837-H11C-8409
GGAATGAGCCATTTACAATTGAATATATCCTGGTGCTTCGGACATGTAGATTTCTATATT
AGGTTTACCCGCCAATATGTTGGTTTCTACTAGGAGTGAAACACGATCTCCGGTAGAAGC
TAGCACACTCAATTTGCTTGAATATTGATTGATTACAATCACCATACGCCAAAGGATAAA
AACCATGATAAAATATGAAAACCACTACAAGGATGGCAAAAAGAGGTAGACCTGAATTTG
CATCAATAATTGTTCTTGAACTTCAATGTTTTGGGAAATATCTTCACAAATGTGGTCATA
CTTTGATATCTCTTTCCTGAACATATCTTCATAGGAGCCTGTTATGGTCATCAGCTTTGG
CAGTATGTCATCCTAATACCAAGTATCAAGTAAAAGCCTAATGAGTTTTGACTCCAATTC
ATACAATAATCAAGAACGAAAATGATGACTAATCAATAGAAAAAATTCACCTTTCTCTTC
ATCTCTTTGAGCATGTCTTCAAGACCTGCCCGCTGAGCACCAAGATTTTCCAGTTGCCTC
TGTGCAAAAAGGGAAGAAAAAAAGTGAGAATAATGGTTCGTGAAAGCACTTTCCAAACAC
ACACTCGCAGCTTCCAAAAGTGATAGAGATTACCAGACTCTGCTTCAAAGTTCCCACAAT
AGCATCCTCTGTTGCGTCCAGAGACATTATTGGCCTAGCAAGAGTTGGGACAGCAGACTC
TATCTGATGAAGAAAAACCGAANACCACAACATCTATGAATCAGTATACAGATNTAGGTT
TGCTATAGCTGACTCAAGTGTGTGATTTGAATAACCTG
   
Sequence Name 76-K106293-0022-837-H11C-02ae
GenBank Accession LN513406 [GenBank]
Sequencing primer gene specific primer, 02ae: 5'-CCTGAAATCTTTGGGTACAGGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K106293-0022-837-H11C-02ae
CAACCTAAATCTGTATACTGATTCAAGATGTTGTGGTTTTCGGTTTTTCTACTCAGATAG
AGTCTGCTGTCCCAACTCTTGCTAGGCCAATAATGTCTCTGGACGCAACAGAGGATGCTA
TTGTGGGAACTTTGAAGCAGAGTCTGGTAATCTCTATCACTTTTGGAAGCTGCGAGTGTG
TGTTTGGAAAGTGCTTTCACGAACCATTATTCTCACTTTTTTTCTTCCCTTTTTGCACAG
AGGCAACTGGAAAATCTTGGTGCTCAGCGGGCAGGTCTTGAAGACATGCTCAAAGAGATG
AAGAGAAAGGTGAATTTTTTCTATTGATTAGTCATCATTTTCGTTCTTGATTATTGTATG
AATTGGAGTCAAAACTCATTAGGCTTTTACTTGATACTTGGTATTAGGATGACATACTGC
CAAAGCTGATGACCATAACAGGCTCCTATGAAGATATGTTCAGGAAAGAGATATCAAAGT
ATGACCACATTTGTGAAGATATTTCCCAAAACATTGAAGTTCAAGAACAATTATTGATGC
AAATTCAGGTCTACCTCTTTTTGCCATCCTTGTAGTGGTTTTCATATTTTATCATGGTTT
TTATCCTTTGGCGTATGGTGATTGTAATCAATCAATATTCAAGCAAATTGAGTGTGCTAG
CTTCTACCGGAGATCGTGTTTCACTCCTAGTAGAAACCAACATATTGGCGGGTAAACCTA
ATATAGAAATCTACATGTCCGAAGCACCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGT
AATTACCAATTTTTTTTCATTCAAAAATGTAG
Primers used for PCR 12cs - 8474, show primer details
   
Sequence Name 53-K108477-0022-837-H11C-12cs
GenBank Accession LN508180 [GenBank]
Sequencing primer gene specific primer, 12cs: 5'-CCTGAAATCTTTGGGTACAGGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K108477-0022-837-H11C-12cs
CAACCTAAATCTGTATACTGATTCATAGATGTTGTGGTTTTCGGTTTTTCTTCATCAGAT
AGAGTCTGCTGTCCCAACTCTTGCTAGGCCAATAATGTCTCTGGACGCAACAGAGGATGC
TATTGTGGGAACTTTGAAGCAGAGTCTGGTAATCTCTATCACTTTTGGAAGCTGCGAGTG
TGTGTTTGGAAAGTGCTTTCACGAACCATTATTCTCACTTTTTTTCTTCCCTTTTTGCAC
AGAGGCAACTGGAAAATCTTGGTGCTCAGCGGGCAGGTCTTGAAGACATGCTCAAAGAGA
TGAAGAGAAAGGTGAATTTTTTCTATTGATTAGTCATCATTTTCGTTCTTGATTATTGTA
TGAATTGGAGTCAAAACTCATTAGGCTTTTACTTGATACTTGGTATTAGGATGACATACT
GCCAAAGCTGATGACCATAACAGGCTCCTATGAAGATATGTTCAGGAAAGAGATATCAAA
GTATGACCACATTTGTGAAGATATTTCCCAAAACATTGAAGTTCAAGAACAATTATTGAT
GCAAATTCAGGTCTACCTCTTTTTGCCATCCTTGTAGTGGTTTTCATATTTTATCATGGT
TTTTATCCTTTGGCGTATGGTGATTGTAATCAATCAATATTCAAGCAAATTGAGTGTGCT
AGCTTCTACCGGAGATCGTGTTTCACTCCTAGTAGAAACCAACATATTGGCGGGTAAACC
TAATATAGAAATCTACATGTCCGAAGCACCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 37-K108476-0022-837-H11C-8409
GenBank Accession LN502643 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K108476-0022-837-H11C-8409
CATTTACAATTGAATATATCCTGGTGCTTCGGACATGTAGATTTCTATATTAGGTTTACC
CGCCAATATGTTGGTTTCTACTAGGAGTGAAACACGATCTCCGGTAGAAGCTAGCACACT
CAATTTGCTTGAATATTGATTGATTACAATCACCATACGCCAAAGGATAAAAACCATGAT
AAAATATGAAAACCACTACAAGGATGGCAAAAAGAGGTAGACCTGAATTTGCATCAATAA
TTGTTCTTGAACTTCAATGTTTTGGGAAATATCTTCACAAATGTGGTCATACTTTGATAT
CTCTTTCCTGAACATATCTTCATAGGAGCCTGTTATGGTCATCAGCTTTGGCAGTATGTC
ATCCTAATACCAAGTATCAAGTAAAAGCCTAATGAGTTTTGACTCCAATTCATACAATAA
TCAAGAACGAAAATGATGACTAATCAATAGAAAAAATTCACCTTTCTCTTCATCTCTTTG
AGCATGTCTTCAAGACCTGCCCGCTGAGCACCAAGATTTTCCAGTTGCCTCTGTGCAAAA
AGGGAAGAAAAAAAGTGAGAATAATGGTTCGTGAAAGCACTTTCCAAACACACACTCGCA
GCTTCCAAAAGTGATAGAGATTACCAGACTCTGCTTCAAAGTTCCCACAATAGCATCCTC
TGTTGCGTCCAGAGACATTATTGGCCTAGCAAGAGTTGGGACAGCAGACTCTATCTGATG
AAGAAAAACCGAANACCACAACATCTATGAATCAGTATACAGATTTAGGTTTGCTATAGC
TGACTCAAGTGTGTGATTTGAATAACCTGTACCC

Last Updated on 06.12.2018 16:11