Sequences from confirmation amplicon


Confirmation sequences for line 839H06 and hit At5g61380 are shown.
Line ID 839H06
Locus At5g61380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24675143
Primers used for PCR BF33 - 8474, show primer details
   
Sequence Name 05-K029007-022-839-H06C-8409
GenBank Accession LN488224 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K029007-022-839-H06C-8409
TCCTCATCTCACCGCCGGCGTTACGCCGGATCTCCGGAGACTTTTGTTGAGCTTTTTCGT
CGATTCATGATTCGATATAGAGAAAAAAGAATTTCCTATTTCAGCTCTTTGTTCTTAGAT
TTATCTTTTTGTTTGTTCTTATGGATAAAGGATTAGGTTGGGGGATTAAAAGAGTAGTTT
TTGTTTGGTTGTGAGATTACTTGACTTTGTTAAGGGGATAAATTAGGCGACTTTGTAGGG
ACACTGTACCGAAAAACCCTAGTTCTGATTTGGCCATGGAAGTATCATAGCACTTACTAT
TGTCGGCTTTACTTCTTCTACTTTTGTATAATCCTTTTTTTTCTTAGGTTCTGTGATTAG
ATATTTTTTTTGTGTGGGTTTAGTTGTTAATCTGATCATGGATTTGAACGGTGAGTGTAA
AGGAGGAGATGGGTTTATTGATAGAAGCAGAGTCAGGATTTTGCTTTGTGACAATGATTC
CACGAGTTTGGGAGAGGTTTTTACTCTCCTTTCAGAGTGTTCTTATCAAGGTATGATTTA
TAGGTTTGTCCTTGTCTTGTT
   
Sequence Name 06-K029007-022-839-H06C-BF33
GenBank Accession LN488676 [GenBank]
Sequencing primer gene specific primer, BF33: 5'-AAGAGAAACTTTGGGAACAAGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K029007-022-839-H06C-BF33
ACAAAGCAAAATCCCGACTCTGCTTTCTATCAATAAACCCATCTCCTCCTTTACACTCAC
CGTTCAAATCCATGATCAGATTAACAACTAAACCCACACAAAAAAAATATCTAATCACAG
AACCTAAGAAAAAAAAGGATTATACAAAAGTAGAAGAAGTAAAGCCGACAATAGTAAGTG
CTATGATACTTCCATGGCCAAATCAGAACTAGGGTTTTTCGGTACAGTGTCCCTACAAAG
TCGCCTAATTTATCCCCTTAACAAAGTCAAGTAATCTCACAACCAAACAAAAACTACTCT
TTTAATCCCCCAACCTAATCCTTTATCCATAAGAACAAACAAAAAGATAAATCTAAGAAC
AAAGAGCTGAAATAGGAAATTCTTTTTTCTCTATATCGAATCATGAATCAACAAAAAAGC
TCAACAAAAGTCACCGGAGATCCGGCGTAACGCCGGCGGTGAGATGAGGAGGAGAGAGAG
AGAAGGTGAATTTCCGACAAGAAGAGTGAGAAGAGAAAGAAAAAGAGTATAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCT

Last Updated on 06.12.2018 16:11