Sequences from confirmation amplicon


Confirmation sequences for line 841E09 and hit At5g51060 are shown.
Line ID 841E09
Locus At5g51060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20760736
Primers used for PCR BF52 - 8474, show primer details
   
Sequence Name 59-K029918-022-841-E09C-8409
GenBank Accession LN509892 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K029918-022-841-E09C-8409
TTGGAAATATTTGTAGGTGAAGAGTATAGCCATAACTATGAACCATAGCACTATAACCCA
ACATCTTTGCCAGTTGTCTAACAAGAAGAATCTAAGACCACGGTACCATCGCTTCAACGG
GTTGCGGTTAAACGTAGGCTTAAGCCTCTGACTCATCATATGACTCAGATTCTTTCTCTC
CCCAGTACTTGTTATCACAGACTGTGTTGCCGCTTGCAAAAGCAGAGTCTCAAGACTCTC
CAACTGTTTCAAGATTCACCACAAATGCTTCAAGTCTCAAACATAATATGGCAAATCGTC
AAGACAGCTGATAATTACTTACCATGATGTATCCTATATTGTCTGGATCCAACTCTTCCA
TGATCAATGCTGCATATTCATCAGCTCTCTTCTGTATTGTAGATAGATTATTCGCAGAAG
   
Sequence Name 59-K029918-022-841-E09C-BF52
GenBank Accession LN509893 [GenBank]
Sequencing primer gene specific primer, BF52: 5'-TTATCAGTCTTAGTGCTTCTGCGA-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K029918-022-841-E09C-BF52
TATGCAGCATTGATCATGGAAGAGTTGGATCCAGACAATATAGGATACATCATGGTAAGT
AATTATCAGCTGTCTTGACGATTTGCCATATTATGTTTGAGACTTGAAGCATTTGTGGTG
AATCTTGAAACAGTTGGAGAGTCTTGAGACTCTGCTTTTGCAAGCGGCAACACAGTCTGT
GATAACAAGTACTGGGGAGAGAAAGAATCTGAGTCATATGATGAGTCAGAGGCTTAAGCC
TACGTTTAACCGCAACCCGTTGAAGCGATGGTACCGTGGTCTTAGATTCTTCTTGTTAGA
CAACTGGCAAAGATGTTGGGTTATAGTGCTATGGTTCATAGTTATGGCTATACTCTTCAC
CTACAAATATATCCAATACACAGGGCTACATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11