Sequences from confirmation amplicon


Confirmation sequences for line 852A11 and hit At1g77450 are shown.
Line ID 852A11
Locus At1g77450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29100228
Primers used for PCR 47bk - 8474, show primer details
   
Sequence Name 86-K107706-0022-852-A11C-47bk
GenBank Accession LN514662 [GenBank]
Sequencing primer gene specific primer, 47bk: 5'-GGCATCTACGTAATTAAACGGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K107706-0022-852-A11C-47bk
CGACGTTGCTCCGTGAATTCCGGTGACACCGCTTGCTCCGAGCCGGAGATCAATCTCTCC
ACAGATTCCGGTGGACACGTGTCAGTCACAGGCTTTAACCCGTCCTCGATATCGCTTCGT
CGCTTCTCGATGACACCTTTCTTGTTATATATACGACACAATACCCAATCGTCCAACTGT
TCAAGAATATCAAAAACACGTCCGGTTAACTAAACCGATTCAAAATCATATTCAAAGTTC
GGTTTGAACTGATAAATTCACTCACTCTTAGACTGTTTTTCTTACGAACCGACCGGTCAA
CGTCAGCGAGCCGGTATTCGTGCATAATCCAATTGGTTTTCTCTCCATTTGGAGGTTTTC
CCGAGTAAAACACTAGAGCCTTCTTAATACCAACCGGTTTAGGACGACCTATTGGTTTAT
CAGCTCCGGTAGCTTTCCAATATCCAGTACCAGCTGCACGGTTGGGTCTTGAACCGTTTG
GATACTTTCGATCTCTTGGTGAGAAAAAATACCACTCCTTTTCACCGTACAAAGCCATGT
CTGTAAATTGTAAACCGGTTTATTAATTCTTAGACCGATTTTATAGAATCCTGTTACTGT
TAGTGTATAGTGTGATTTAAAGGGGAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGGTTATTA
   
Sequence Name 70-K107705-0022-852-A11C-8409
GenBank Accession LN512403 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K107705-0022-852-A11C-8409
TCCTTTATCCACTATACCTAACAGTAACAGGATTCTATAAAATCGGTCTAAGAATTAATC
ATCCGGTTTACAATTTACAGACATGGCTTTGTACGGTGAAAAGGAGTGGTATTTTTTCTC
ACCAAGAGATCGAAAGTATCCAAACGGTTCAAGACCCAACCGTGCAGCTGGTACTGGATA
TTGGAAAGCTACCGGAGCTGATAAACCAATAGGTCGTCCTAAACCGGTTGGTATTAAGAA
GGCTCTAGTGTTTTACTCGGGAAAACCTCCAAATGGAGAGAAAACCAATTGGATTATGCA
CGAATACCGGCTCGCTGACGTTGACCGGTCGGTTCGTAAGAAAAACAGTCTAAGAGTGAG
TGAATTTATCAGTTCAAACCGAACTTTGAATATGATTTTGAATCGGTTTAGTTAACCGGA
CGTGTTTTTGATATTCTTGAACAGTTGGACGATTGGGTATTGTGTCGTATATATAACAAG
AAAGGTGTCATCGAGAAGCGACGAAGCGATATCGAGGACGGGTTAAAGCCTGTGACTGAC
ACGTGTCCACCGGAATCTGTGGCGAGATTGATCTCCGGCTCGGAGCAAGCGGTGTCACCG
GAATTCACGTGTAGCAACGGTCGGTTGAGTAATGCCCTTGATTTTCCGTTTAATTACGTA
GATGC

Last Updated on 06.12.2018 16:11