Sequences from confirmation amplicon


Confirmation sequences for line 860G07 and hit At5g62720 are shown.
Line ID 860G07
Locus At5g62720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25193519
Primers used for PCR 21nt - 8474, show primer details
   
Sequence Name 71-K114716-0022-860-G07C-21nt
GenBank Accession LN512605 [GenBank]
Sequencing primer gene specific primer, 21nt: 5'-ACGAGTAATGACCATAAAAGCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K114716-0022-860-G07C-21nt
TCGGGCGAGCAGCCTGGGCCGAAACGGAGAAAGCACCACCCCAATCGCAGCACAACCTAT
TTGAGCCAAAAATATATTGTATTTCTGAAAATATAAGAAACACAAAACACACTCCTCAAT
TAATCAGTTAAATAACTGATACGATGACAAATTACCAATTACTAGTACTTCAAGTTTCTA
TTTTAACTTGATGTGATGGCATCTCTTTCCAATCTAAACATGAGAAGATGGCTGAAATAG
GTTGTGACTAATTATCCAAATATATGAAAGTATCAATCAACGATAAAATAGTGGACCTCT
AGATTTATTCCATAGAAAAGTGTAAGCACAAGAACAAAATTAGAAGAAAATCTTCAACTA
GCTTATGGATATAGAACTTAGTGGGAAAATGTCTCCTCTAACCACTACTTTCTACTTTTG
ATTATTCAATTCCTCTATGTTTGTTACACACCATCCAAATCCTAACAACTCACTTTTGGT
ATTTGCATTTTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCACAA
   
Sequence Name 23-K114715-0022-860-G07C-8409
GenBank Accession LN496661 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K114715-0022-860-G07C-8409
AAATGCAAATACCAAAAGTGAGTTGTTAGGATTTGGATGGTGTGTAACAAACATAGAGGA
ATTGAATAATCAAAAGTAGAAAGTAGTGGTTAGAGGAGACATTTTCCCACTAAGTTCTAT
ATCCATAAGCTAGTTGAAGATTTTCTTCTAATTTTGTTCTTGTGCTTACACTTTTCTATG
GAATAAATCTAGAGGTCCACTATTTTATCGTTGATTGATACTTTCATATATTTGGATAAT
TAGTCACAACCTATTTCAGCCATCTTCTCATGTTTAGATTGGAAAGAGATGCCATCACAT
CAAGTTAAAATAGAAACTTGAAGTACTAGTAATTGGTAATTTGTCATCGTATCAGTTATT
TAACTGATTAATTGAGGAGTGTGTTTTGTGTTTCTTATATTTTCAGAAATACAATATATT
TTTGGCTCAAATAGGTTGTGCTGCGATTGGGGTGGTAGCTTTCTCCGTCTTCGGCCCAGG
CTGGCTCGCCCGGAGTGTCGCCCTCGCCGCTTCCATCGCTTTTATGGTCATTACTCG

Last Updated on 06.12.2018 16:11