Sequences from confirmation amplicon


Confirmation sequences for line 863D07 and hit At1g12140 are shown.
Line ID 863D07
Locus At1g12140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4121771
Primers used for PCR TH32 - 8474, show primer details
   
Sequence Name 67-K103678-0022-863-D07C-TH32
GenBank Accession LN511797 [GenBank]
Sequencing primer gene specific primer, TH32: 5'-ATAGTTGGCGAATAAGAAAAAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K103678-0022-863-D07C-TH32
ATTTTAGTATCAGTAAAAGGGTATGGACAAAAGATAAAGATTAAAAAACTAAAATTTAGT
AAACTGTTCACATATTCTGCAAGAACGTACCTGGTCTTTGAATTGATCAGGAACACGGTA
ATTGTGGCTATGAATCTGCTTCCCTGGCCATGAATCTATACCTAAAATAACAAGTTTTCT
CATCAATCAAAACATATTCACTTCAAAATCATTCAATCTAAAAAAACATCTGAAATTGAA
GTACCAGGAACATGAGCAACTCTAGGTTCTGTATAATGTCCATTACAAACAACAACAGCA
TCAAAGATCTCATCTTTGGAGATCCCATCTGAATTTTTGGATTGAACCCTCCATTTCTTA
TCTTCAGGCTCGACAAGAACTACTTCGGTCTTAAATCGAACCATCTGTGATGACGTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 71-K103679-0022-863-D07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K103679-0022-863-D07C-8409
CGTCATCACAGAATGGGGTTCGATTTAAGAACCGAAGTTAGTTCTTGGTCGANNGCCTGA
AGATAANGAAATGGGGAGGGTNNTCAATCCAAAAATTCAGAAATGGGANNTCTCCAAAGA
TGAAGATNNCTTTGATGCNTNNGTTGTTNNGTTTGTAATGGGGACATTANNTACAGANNA
CCTAGANNGTTGCTCATGGGTTCCTNGGGTACTTCAATTTTTCAGAANTGTTTTTTNNTA
GATTGAANNTGATTTTGNNAAGTGAATATGNNNTTTTGATNNTGATGAGAANNAACTTGT
TNNATTTTAGGTANNTAGATTCATGGGGCCAGGGNGAAGCAGAAATTCATAGCCANNCAA
TTACCGTGGTTNNCCTGATCANNATTCAAAGAACCAGGGTACGNTTNTCTTGCAGAAAAT
ATGTNNGAACAGTTTNNACTAAATTTTNNAGTTTTTTANNATCTTTATNCNNTTTTGTCN
NCATACC

Last Updated on 06.12.2018 16:11