Sequences from confirmation amplicon


Confirmation sequences for line 868F07 and hit At1g31380 are shown.
Line ID 868F07
Locus At1g31380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11240519
Primers used for PCR 36oc - 8474, show primer details
   
Sequence Name 40-K114678-0022-868-F07C-36oc
GenBank Accession LN503800 [GenBank]
Sequencing primer gene specific primer, 36oc: 5'-CTCATTTGTGTGCTAACCAACTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K114678-0022-868-F07C-36oc
ACACGTTTTTTTATTACCAAGTCTCTTGATCTTCTACTCAAAGATTAAGAGGACATTGTT
CTCAAAACTAAAACGAAAGAGAAACATCCCAGAACACCAATTAGATCCAAAGCTTTAACA
GACTATAATCTACTCAAGTCATCCTCATCGTCAGAAACCCAGCAGATATTTTGTAAAAAC
AACAGTCAAACAACAAATTTAAGGACAAAACTATATAAAAAAGCTTGATATGACTCTTTA
TTGATAAGGGCACATACGTATCATCAGCACTGATTGAGACAGGAAAGACATTTTTAAATT
CCTTTTCCATCAAGAAACAGTGTGAAAATAAAGAAAATTAGGGCAACATAAATAACAAGT
CTCTTTTACTTATCCAAGATTAAGAGGATTCTATTCCAAAACTAAAACGAAAACAAAAGA
GACCAAAACACCATTCATAGCAACTCAAGTCATCAAACAACATTCATAGTAAGAACTCCA
GAAGAGCCTCCATGTCTACACACTTATGCTTCATCTCTACGCACTTTTGCTTTAACAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAATCCG
   
Sequence Name 16-K114677-0022-868-F07C-8409
GenBank Accession LN493533 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K114677-0022-868-F07C-8409
TCATTGTTAAGCAAAGTGCGTAGAGATGAAGCATAAGTGTGTAGACATGGAGGCTCTTCT
GGAGTTCTTACTATGAATGTTGTTTGATGACTTGAGTTGCTATGAATGGTGTTTTGGTCT
CTTTTGTTTTCGTTTTAGTTTTGGAATAGAATCCTCTTAATCTTGGATAAGTAAAAGAGA
CTTGTTATTTATGTTGCCCTAATTTTCTTTATTTTCACACTGTTTCTTGATGGAAAAGGA
ATTTAAAAATGTCTTTCCTGTCTCAATCAGTGCTGATGATACGTATGTGCCCTTATCAAT
AAAGAGTCATATCAAGCTTTTTTATATAGTTTTGTCCTTAAATTTGTTGTTTGACTGTTG
TTTTTACAAAATATCTGCTGGGTTTCTGACGATGAGGATGACTTGAGTAGATTATAGTCT
GTTAAAGCTTTGGATCTAATTGGTGTTCTGGGATGTTTCTCTTTCGTTTTAGTTTTGAGA
ACAATGTCCTCTTAATCTTTGAGTAGAAGATCAAGAGACTTGTGTAATAAAAAAAACGTG
TTAGTGTCCTTAAATCTGAACTGTGACAGTTGGTTAGCAACCAAATGA

Last Updated on 06.12.2018 16:11