Sequences from confirmation amplicon


Confirmation sequences for line 892H04 and hit At3g19500 are shown.
Line ID 892H04
Locus At3g19500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6760310
Primers used for PCR NA33 - 8474, show primer details
   
Sequence Name 25-K101563-0022-892-H04C-8409
GenBank Accession LN497377 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K101563-0022-892-H04C-8409
GCCATTTACAATTGAATATATCCTGATGATTTCTCTTGATCCATATACATACTATTAAAT
GTCACACCTTTGCAATTCCAACTGATGATTTCTCTTGATTTCGTTTCCCTTTCTTGCCCG
GTTTTCGATTTTGATCCGACTCTTGCCCATTACCCGTTCCGGTTCTCTTCTTCTTCACAA
GACACACACAATAAAAAACACATCTTTAAATTTGTAATCCTTAATTACATGCAACTATGC
AAATAAAGAATCAGTAATGAGAGACTTACGGTTGATGATTTAGTAGATTTATCGACCGTA
CACGCGTCGTTTGAGTTCCTGCAATCAATCTTAATGCATGAAGGGTCAAGATCAGAGACT
CCGGAGGTTGGAACAGAAGGTTCTAGGAAGAGTGACTCGTCGTCGTTTTGATAGCCTCCA
CTAAAACAGAGCATTCTTGAGCCAGTTTTGCCGGAGAATACAAAACCTAAACTGTCTTCT
CCACC
   
Sequence Name 25-K101576-0022-892-H04C-NA33
GenBank Accession LN497378 [GenBank]
Sequencing primer gene specific primer, NA33: 5'-TGGTGGAGAAGACAGTTTAGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K101576-0022-892-H04C-NA33
AGATGCTCTGTTTAGTGGAGGCTATCAAAACGACGACGAGTCACTCTTCCTAGAACCTTC
TGTTCCAACCTCCGGAGTCTCTGATCTTGACCCTTCATGCATTAAGATTGATTGCAGGAA
CTCAAACGACGCGTGTACGGTCGATAAATCTACTAAATCATCAACCGTAAGTCTCTCATT
ACTGATTCTTTATTTGCATAGTTGCATGTAATTAAGGATTACAAATTTAAAGATGTGTTT
TTTATTGTGTGTGTCTTGTGAAGAAGAAGAGAACCGGAACGGGTAATGGGCAAGAGTCGG
ATCAAAATCGAAAACCGGGCAAGAAAGGGAAACGAAATCAAGAGAAATCATCAGTTGGAA
TTGCAAAGGTGTGACATTTAATAGTATGTATATGGATCAAGAGAAATCATCAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCACGNTTATT

Last Updated on 06.12.2018 16:11