Sequences from confirmation amplicon


Confirmation sequences for line 894A06 and hit At2g21390 are shown.
Line ID 894A06
Locus At2g21390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9155812
Primers used for PCR 27kk - 8474, show primer details
   
Sequence Name 67-K113934-0022-894-A06C-27kk
GenBank Accession LN511862 [GenBank]
Sequencing primer gene specific primer, 27kk: 5'-GACTTGACAAATTCGATGTTGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K113934-0022-894-A06C-27kk
ATGGATGGTGAGAACAGGTGAAGAAAAGACGACGAGGAAGGTGGATGGGGACTTGACTTG
GATCTTCCACCAGAGCTTGACACTCCTAAAGCCTCTGCTAACGCACGTTCATCTACTTTT
GTGACACCTCCTCAAGGTATGCCCGTAAGTCAGATTTGGAGTCAGAAATCTTCCCTTGCT
GCTGAGCAGGCTGCAGCAGGAAGCTTCGACACTGCAATGCGTTTGCTCCACAGGCAGCTT
GGCATTAAGAACTTTGCGCCTTTGAAATCGATGTTTCTTGATCTGTTCAGTGGGAGCCAT
AGCTATTTATAATTACTCTTACTTTTTCGCCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 32-K113933-0022-894-A06C-8409
GenBank Accession LN500667 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K113933-0022-894-A06C-8409
CATTTCATTGAATATATCCTGGCGAAAAGTAAGAGTAATTATAAATAGCTATGGCTCCCA
CTGAACAGATCAAGAAACATCGATTTCAAAGGCGCAAAGTTCTTAATGCCAAGCTGCCTG
TGGAGCAAACGCATTGCAGTGTCGAAGCTTCCTGCTGCAGCCTGCTCAGCAGCAAGGGAA
GATTTCTGACTCCAAATCTGACTTACGGGCATACCTTGAGGAGGTGTCACAAAAGTAGAT
GAACGTGCGTTAGCAGAGGCTTTAGGAGTGTCAAGCTCTGGTGGAAGATCCAAGTCAAGT
CCCCATCCACCTTCCTCGTCGTCTTCTTCTTCACCTGCTTCTGCACCATCCAATATGGCT
TCGATGTCTGTGTTCTCCATTCCATCAACATCGAATTTGTCAAGT

Last Updated on 06.12.2018 16:11