Sequences from confirmation amplicon


Confirmation sequences for line 894G04 and hit At5g14490 are shown.
Line ID 894G04
Locus At5g14490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4671669
Primers used for PCR 30kk - 8474, show primer details
   
Sequence Name 70-K113845-0022-894-G04C-30kk
GenBank Accession LN512427 [GenBank]
Sequencing primer gene specific primer, 30kk: 5'-AGCAAAAACCAAACACTGAAAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K113845-0022-894-G04C-30kk
GCCTAAGCATCTAGAGAAATTTTTAGTGATTCTTGAAATCTTTGTCTGTTTTTGACAAGA
AACGCTTGAATCTAGTACCACAAATATGTTTCTCCGTCTTCTTAAACCTTGTATATATAG
CTTTGCTATTGACACTAAACCGATGAAAACTCTACACAGGACTTGGATAGTGGATGGCCC
ATGGATCTCAAGGAATGTAAAGAATGCGTCTCTGTCTTCAGCTCTGCAGATTAAAGACTG
CGGAGCTTATATCAACTGCTTTAACTGCTCTTACCGCATCGACAACAGCAATGTAAAGTA
ACAAATATAGAACACATGTAACTAGAGCTTATGGCCCTGTTTATGTGAATACTGAGATAG
TATTTTTCTTATTTTGCTTTGTCAGGTACTGACTCCATGGCCAGGCCTTCCTAAAGGTGT
GAAATTCGAGCCAACCGATGAGGAAGTGATTGAGCATTTGGAAGCTAAGTGTGGTATTGA
TGGCTTAAAACCACATCTTCTTATCCAAGACTTTATATGTTCAGTCACTCAAGATGTTGG
TATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCC
   
Sequence Name 43-K113844-0022-894-G04C-8409
GenBank Accession LN504909 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K113844-0022-894-G04C-8409
TTACATTGATACCAACATCTTGAGTGACTGAACATATAAAGTCTTGGATAAGAAGATGTG
GTTTTAAGCCATCAATACCACACTTAGCTTCCAAATGCTCAATCACTTCCTCATCGGTTG
GCTCGAATTTCACACCTTTAGGAAGGCCTGGCCATGGAGTCAGTACCTGACAAAGCAAAA
TAAGAAAAATACTATCTCAGTATTCACATAAACAGGGCCATAAGCTCTAGTTACATGTGT
TCTATATTTGTTACTTTACATTGCTGTTGTCGATGCGGTAAGAGCAGTTAAAGCAGTTGA
TATAAGCTCCGCAGTCTTTAATCTGCAGAGCTGAAGACAGAGACGCATTCTTTACATTCC
TTGAGATCCATGGGCCATCCACTATCCAAGTCCTGTGTAGAGTTTTCATCGGTTTAGTGT
CAATAGCAAAGCTATATATACAAGGTTTAAGAAGACGGAGAAACATATTTGTGGTACTAG
ATTCAAGCGTTTCTTGTCAAAAACAGACAAAGATTTCAAGAATCACTAAAAATTTCTCTA
GATGCTTAGGCTATGAATGGCAATAGTTCTCAAAATGAGTTTCAAGTTTTCAGTGTTGNT
TTTTTTG

Last Updated on 06.12.2018 16:11