Sequences from confirmation amplicon


Confirmation sequences for line 910B06 and hit At2g24290 are shown.
Line ID 910B06
Locus At2g24290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10339799
Primers used for PCR 82ns - 8474, show primer details
   
Sequence Name 48-K114664-0022-910-B06C-82ns
GenBank Accession LN506581 [GenBank]
Sequencing primer gene specific primer, 82ns: 5'-TTGTTAGTGACTCTTGACCCACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K114664-0022-910-B06C-82ns
ATCCACATTAGCTGCAAACAAATCCTCTTACAAATCAAATCAGGGCTTGCCCTCTCTAAT
CAGATGCACATCTCAAAAAACACAATATCCAAGTTCTATAAGATCATAATTATATATTTT
GCTTTTGCCTGGTGAGAAAATGATGGTTGCGTTTGCCAATAAACATGAACAATAAAATAC
AGAAGTCGGTGACTTTGTCCAGTGTGACTGACAAATATATATCTGAAGAAAAAAGTCAAT
ACAAGGCCAACCCTTTCTTTAGCAGTGTCTAGAGCCAAGAGCAAAACAAAAAAGAACTAC
ATATATAGAGAACATGTGAGACAAAAACATTGCCTTGCGAATTAAGCGATTCCCATGTTC
TCACGAGTTGTATACGGAAGAGAAAATCTAGTTTGCTTCCATTGAGAAACTCGTCAGAGT
AGACTGTGAAGAAGTCACTAAACAGTGAATTACTTACTCACCTTCCCAACCTAATTGCCG
AGCTCTGCGCTCCCACAAAAAAGTGATCTTCCGCAATGATCTTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 19-K114663-0022-910-B06C-8409
GenBank Accession LN494869 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K114663-0022-910-B06C-8409
GAGATCATTGCGGAAGATCACTTTTTTGTGGGAGCGCACATCTCGGCAATTAGGTTGGGA
AGGTGAGTAAGTAATTCACTGTTTAGTGACTTCTTCACAGTCTACTCTGACGAGTTTCTC
AATGGAAGCAAACTAGATTTTCTCTTCCGTATACAACTCGTGAGAACATGGGAATCGCTT
AATTCGCAAGGCAATGTTTTTGTCTCACATGTTCTCTATATATGTAGTTCTTTTTTGTTT
TGCTCTTGGCTCTAGACACTGCTAAAGAAAGGGTTGGCCTTGTATTGACTTTTTTCTTCA
GATATATATTTGTCAGTCACACTGGACAAAGTCACCGACTTCTGTATTTTATTGTTCATG
TTTATTGGCAAACGCAACCATCATTTTCTCACCAGGCAAAAGCAAAATATATAATTATGA
TCTTATTGAACTTGGATATTGTGTTTTTTGAGATGTGCATCTGATTGAGACAGCAAGCCT
GATTTGATTTGTAAGAGGATTTGTTTTGCAGCTAATGTTGGATGCATCTGTGATTATGGT
TTAAGCATAAGTGTGGGTCAA

Last Updated on 06.12.2018 16:11