Sequences from confirmation amplicon


Confirmation sequences for line 929A02 and hit At5g06780 are shown.
Line ID 929A02
Locus At5g06780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2096582
Primers used for PCR 45qz - 8474, show primer details
   
Sequence Name 46-K116132-0022-929-A02C-45qz
GenBank Accession LN505968 [GenBank]
Sequencing primer gene specific primer, 45qz: 5'-CCAATCAGCTCTTTATAGCCTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K116132-0022-929-A02C-45qz
TTGTACTTAGTTTCGAGCGATTTAAGAACATTTCTCCTTTTCGTCCATTCGAGAGAATCC
TAACAGTTCTAGGGTTTTGAAATATCACTGGAGTTTAAAAAAAAGGGTTTTACTAAATTA
TTCTCTTTCTTAGGGTTTAAGATATTCTTTTCACATTCTATTAGATTGTTTGATCATCTC
ATGTCTATTTTTTTTGCTGTCCGAATATTCGCTTAAACTAAAGTTATCCAATTTCTTGAT
ACAATAAGTCCACCATTCAGCAATGAGTATGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 21-K116131-0022-929-A02C-8409
GenBank Accession LN495806 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K116131-0022-929-A02C-8409
GAAAATCCGGAATCACTTTCGTTTAAGCGAAATATTCCGGAACAGCAAAAAAAATAGACA
TCGAGATGATCAAACAATCTAATAGAATGTGAAAAGAATATCTTAAACCCTAAGAAAGAG
AATAATTTTAGTAAAACCCTTTTTTTTAAACTCCAGTGATATTTCAAACCCTAGAACTGT
TAGATCTCTCAATGGACAAAAGAGAAATGTCTTAAATCGCTCAAACTAAGTACAAAACAG
AAATTATACCTCCTTATTCAGAATCCTGTCGTAAAACAGCAATCTTTATACGAAACCCTT
AATTGTCGAAGAGAGAAAGATTGACTCCCAAACGCAAAGGCTAT

Last Updated on 06.12.2018 16:11