Sequences from confirmation amplicon


Confirmation sequences for line 934E06 and hit At4g03350 are shown.
Line ID 934E06
Locus At4g03350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1474686
Primers used for PCR NF62 - 8474, show primer details
   
Sequence Name 39-K102502-0022-934-E06C-8409
GenBank Accession LN503309 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K102502-0022-934-E06C-8409
ATTTACATTGAATAATCCTGCGACTAATAACCGTTGGATCATATCAGTAAGGAGAGAAAT
CGACGGTTAGAATTCTTTGACTAGTGATATTTAAGCGGCCAAATCACGAAATTAACAAAC
TTTCGTCACAAATCATCAGTTTCTCTTTCTCTCTTCCTCCTTAGTCTCTCTCCACTGATC
TTGAAACTACAACGATGAACGTGGACATCGATACTGAGACTGGCTCATCGTTCTCCATTA
CTATTGATTTCGGCGAAACAGTGTTACAGATAAAAGAGAAGATCGAGAAGTCTCAAGGTA
TCCCTGTTTCGAAACAAATCCTTTACTTGGATGGCAAGGCTCTTGAAGATGATCTTCATA
AGATTGATTACATGATCCTCTTCGAATCTCTCCTTCTCCGCATCTCTCCTGATGCTGACC
CTAATCAGAGCAACGAGCAAACCGAGCAATCAAAACAAATTGATGATAAGAAACAAGAGT
TCTGTGGCATTCAAGATTCATCGGAGAGCAAAAAGCTCACGAGGGTGATGGCGAGGAGAG
TCCACAATGTATATTCATCACTACCGGCTTATTCACTTGATGAGTTACTTGGCCCTAAAT
ATTCAGCAACTGTGACGGTTGGAGGCAGAACAAATCAAGTGGTTCAAACCACCGAGCAAG
CTTCAACGTCAGGCACAGCCAAAGAGGTCCTTAGAGATTCAGATTCGCCTGTGGAAAAGA
AGATCAAGACAAACCCGATGAAGTTTACAGTACATGTGAAGCCATATCAAGAAGATACCA
AGATGATTCAAGTAGAGGTTAACGCAGATGACAACGTTGAAGAACTGAGGAAAGAGCTGG
TTAAGATGCAAG
   
Sequence Name 39-K102503-0022-934-E06C-NF62
GenBank Accession LN503310 [GenBank]
Sequencing primer gene specific primer, NF62: 5'-CAGATTTAACTCACCCCTCTCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K102503-0022-934-E06C-NF62
ACGTTGTCATCTGCGTTAACCTCTACTTGAATCATCTTGGTATCTTCTTGATATGGCTTC
ACATGTACTGTAAACTTCATCGGGTTTGTCTTGATCTTCTTTTCCACAGGCGAATCTGAA
TCTCTAAGGACCTCTTTGGCTGTGCCTGACGTTGAAGCTTGCTCGGTGGTTTGAACCACT
TGATTTGTTCTGCCTCCAACCGTCACAGTTGCTGAATATTTAGGGCCAAGTAACTCATCA
AGTGAATAAGCCGGTAGTGATGAATATACATTGTGGACTCTCCTCGCCATCACCCTCGTG
AGCTTTTTGCTCTCCGATGAATCTTGAATGCCACAGAACTCTTGTTTCTTATCATCAATT
TGTTTTGATTGCTCGGTTTGCTCGTTGCTCTGATTAGGGTCAGCATCAGGAGAGATGCGG
AGAAGGAGAGATTCGAAGAGGATCATGTAATCAATCTTATGAAGATCATCTTCAAGAGCC
TTGCCATCCAAGTAAAGGATTTGTTTCGAAACAGGGATACCTTGAGACTTCTCGATCTTC
TCTTTTATCTGTAACACTGTTTCGCCGAAATCAATAGTAATGGAGAACGATGAGCCAGTC
TCAGTATCGATGTCCACGTTCATCGTTGTAGTTTCAAGATCAGTGGAGAGAGACTAAGGA
GGAAGAGAGAAAGAGAAACTGATGATTTGTGACGAAAGTTTGTTAATTTCGTGATTTGGC
CGCTTAAATATCACTAGTCAAAGAATTCTAACCGTCGATTTCTCTCCTTACTGATATGAT
CCAACGGTTATTAGTCGCAGGATATATTCAATTGNTAATGGCTTCATGTCGGGGAAATCT
ACATGGGATCAGCATGAGTATGATGGNTCATATGGAGAAAAAGAA

Last Updated on 06.12.2018 16:11