Sequences from confirmation amplicon


Confirmation sequences for line 936E04 and hit At1g73650 are shown.
Line ID 936E04
Locus At1g73650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27688611
Primers used for PCR 87ra - 8474, show primer details
   
Sequence Name 48-K116132-0022-936-E04C-87ra
GenBank Accession LN506607 [GenBank]
Sequencing primer gene specific primer, 87ra: 5'-TTTTTATTTGCCTGTGTATTCGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K116132-0022-936-E04C-87ra
ACATATAAAGTAATTCAATAATTCAAATAATCAAATCCGAAGTCGTCCAGCGGTTAGGAA
TATTTCGGCTTTCACCCAGGAGACCCGGAGTTCGATTCTTGGAATCGGGAACTTTTTTTA
ACCAAAAAACAAAATCACATAAAAAAAAGAACCAAATACAACTGAGATAATCTGTCAAGA
CTGTCCAATATTTCAAACAAATTCAGTTTCTTACGGGCAATTTTTTACACAAATTTTATC
AAAGAGTGTTGTGAAGAACAATAACTCAAATATGAGTATCACAAATCAGTCATCTTTTAC
TTCTTTCTCTTTGCTGTTTTGGAGTCTACCCTAACTTTCTTGCACACATACAACAAAGAG
AAGCCAAACTCATCAAACACTAAAGTAAGATATAAAAGATGAAACTGAAACAGAATAAGC
AGTTACTAAACAGCCACCTCTTGAGGGAGATTTCGGCTGTAAAATGGAAACTCGAAGAGA
AAGACCGTCTTGAACCATCCTGGTAAGTTCCCATACACTCCTCTTAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
   
Sequence Name 23-K116131-0022-936-E04C-8409
GenBank Accession LN496699 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K116131-0022-936-E04C-8409
GAACGGTCTTTTCTTCTTCCGAGTTTTCCATTTTACAGCCGAAATCTCCCTCAAGAGGTG
GCTGTTTAGTAACCGCTTATTCTGTTTCAGTTTCATCTTTTATATCTTACTTTAGTGTTT
GATGAGTTTGGCTTCTCTTTGTTGTATGTGTGCAAGAAAGTTAGGGTAGACTCCAAAACA
GCAAAGAGAAAGAAGTAAAAGATGACTGATTTGTGATACTCATATTTGAGTTATTGTTCT
TCACAACACTCTTTGATAAAATTTGTGTAAAAAATTGCCCGTAAGAAACTGAATTTGTTT
GAAATATTGGACAGTCTTGACAGATTATCTCAGTTGTATTTGGTTCTTTTTTTTATGTGA
TTTTGTTTTTTGGTTAAAAAAAGTTCCCATTCCAAGAATCGAACCCGGGTCTCCTGGGTG
AAAGCCAAATATCCTAACCGCTGGACGACATCGGATTTGATTATTTGATTATTTAATTAC
TTTATATGTTGATTTTCATATTTATTTGGATTTAATTGTATGATTTAAATTCATGTACTC
AACCTAGGCAAAATTTTCACGAAT

Last Updated on 06.12.2018 16:11