Sequences from confirmation amplicon


Confirmation sequences for line 951C02 and hit At3g23250 are shown.
Line ID 951C02
Locus At3g23250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8310362
Primers used for PCR 46lx - 8474, show primer details
   
Sequence Name 83-K114129-0022-951-C02C-46lx
GenBank Accession LN514340 [GenBank]
Sequencing primer gene specific primer, 46lx: 5'-GATGCTATCATCAGCTTACACCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K114129-0022-951-C02C-46lx
AACCGACACACAAGTTTTATTTTGTTTTCTTACTATATATAAATCACCAAAAGAAAGTAA
CTTTATACACACGTCTAGGTCGCACTACTATTCTTTTAACTACACAATGATCGGCTCTTT
AATTACTTTTGTATTGATGTTCTTCGTTTTCCTTTTGGTATCATCTTTAGATGGTCAGCG
ATTGCAGCAAAACTGCCTGGAAGAACCGATAACGAGATCAAGAACGTATGGCACACTCAC
TTGAAGAAGAGACTCGAAGATTATCAACCAGCTAAACCTAAGACCAGCAACAAAAAGAAG
GGTACTAAACCAAAATCTGAATCCGTAATAACGAGCTCGAACAGTACTAGAAGCGAATCG
GAGCTAGCAGATTCATCAAACCCTTCTGGAGAAAGCTTATTTTCGACATCGCCTTCGACA
AGTGAGGTTTCTTCGATGACACTCATAAGCCACGACGGCTATAGCAACGAGATTAATATG
GATAACAAACCGGGAGATATCAGGCGTACATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGGTTATT
   
Sequence Name 41-K114128-0022-951-C02C-8409
GenBank Accession LN504161 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K114128-0022-951-C02C-8409
ACGCCTGAATCTCCCGGTTTGTTTCCATATTAATCTCGTTGCTATAGCCGTCGTGGCTTA
TGAGTGTCATCGAAGAAACCTCACTTGTCGAAGGCGATGTCGAAAATAAGCTTTCTCCAG
AAGGGTTTGATGAATCTGCTAGCTCCGATTCGCTTCTAGTACTGTTCGAGCTCGTTATTA
CGGATTCAGATTTTGGTTTAGTACCCTTCTTTTTGTTGCTGGTCTTAGGTTTAGCTGGTT
GATAATCTTCGAGTCTCTTCTTCAAGTGAGTGTGCCATACGTTCTTGATCTCGTTATCGG
TTCTTCCAGGCAGTTTTGCTGCAATCGCTGACCATCTAAAGATGATACCAAAAGGAAAAC
GAAGAACATCAATACAAAAGTAATTAAAGAGCCGATCATTGTGTAGTTAAAAGAATAGTA
GTGCGACCTAGACGTGTGTATAAAGTTACTTTCTTTTGGTGATTTATATATAGTAAGAAA
ACAAAATAAAACTTGTGTGTCGGTTATATACTTATATGTGAAGTAAAATACCTATTGCCA
AGTATTTGGTGTAAGCTGATGATAGCAT

Last Updated on 06.12.2018 16:11