Sequences from confirmation amplicon


Confirmation sequences for line 955D10 and hit At3g19760 are shown.
Line ID 955D10
Locus At3g19760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6863773
Primers used for PCR AC88 - 8474, show primer details
   
Sequence Name 15-K104337-0022-955-D10C-AC88
GenBank Accession LN492953 [GenBank]
Sequencing primer gene specific primer, AC88: 5'-TTAGGAACGAGATAGGGTGAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K104337-0022-955-D10C-AC88
AGACCGATCTAAGTCTGTAGGTTCTTCTTCTGAAGCTGAAGCTGGGTTCGAAGTTCAGCT
TCGTTGATGCAGTGTTGTCGTTTTGACGCAAAATTGCTACTTGCCTTCCTATGGATATGG
GCCTAACTATAAAGGCCCACTAAACATATAAGAGTCGGTATTTTCCTGACAAAAAAGCCC
TAACAAGTTGTCGGATTTACAATCGTACCCCTATTTTTAAGCCGCGCTTATCTCGAACCT
ATACCTGTAGATTTTCGATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 31-K104338-0022-955-D10C-8409
GenBank Accession LN500145 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K104338-0022-955-D10C-8409
TCGAAATCTACAGGTATAGGTTCGAGATAAGCGCGGCTTAAAAATAGGGGTACGATTGTA
AATCCGACAACTTGTTAGGGCTTTTTTGTCAGGAAAATACCGACTCTTATATGTTTAGTG
GGCCTTTATAGTTAGGCCCATATCCATAGGAAGGCAAGTAGCAATTTTGCGTCAAAACGA
CAACACTGCATCAACGAAGCTGAACTTCGAACCCAGCTTCAGCTTCAGAAGAAGAACCTA
CAGACTTTAGATCGGTCTTTATCTCTCTCTCCTCTGTCGGGATTTTCTCTCTCTTCTTCA
CCCTATCTCGTTCCTA
Primers used for PCR 30ah - 3144, show primer details
   
Sequence Name 05-K106400-0022-955-D10C-3144
GenBank Accession LN488365 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K106400-0022-955-D10C-3144
CCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAG
ATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTACGGCGAAATCAATCTAGACG
GAGATTTATGGCGACAGCGAATCCTGGCCGTGGAGGTGGTAGAAGAGGCGGTGGAGCTAT
GGATGACGATAAGTTGGTCTTCGAGACTACCGATGGAATTGAGCCCATCACGAGCTTCAA
TGATATGGGCATTAAGGAAGATGTGCTTCGCGGTGTCTACGAATATGGATTCGAGAAGCC
TTCCGCGATTCAGCAGAGGGCTGTTATGCCGATTCTTCAAGGACGTGATGTTATTGCTCA
AGCTCAGTCCGGTACCGGGAAGACATCTATGATTGCTCTCTCCGTTTGTCAAGTCGTTGA
TACTTCTTCTAGAGAGTATGTTTTCTGTTCCTTTAGCTGGA
   
Sequence Name 21-K106401-0022-955-D10C-30ah
GenBank Accession LN495673 [GenBank]
Sequencing primer gene specific primer, 30ah: 5'-ATCCAGCTAAAGGAACAGAAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K106401-0022-955-D10C-30ah
AGATGTCTTCCCGGTACCGGACTGAGCTTGAGCAATAACATCACGTCCTTGAAGAATCGG
CATAACAGCCCTCTGCTGAATCGCGGAAGGCTTCTCGAATCCATATTCGTAGACACCGCG
AAGCACATCTTCCTTAATGCCCATATCATTGAAGCTCGTGATGGGCTCAATTCCATCGGT
AGTCTCGAAGACCAACTTATCGTCATCCATAGCTCCACCGCCTCTTCTACCACCTCCACG
GCCAGGATTCGCTGTCGCCATAAATCTCCGTCTAGATTGATTTCGCCGTAAACACTGATA
GTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATG
AAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATC
CCTTACGTCAGTGGAGATATCACATCAATCCA

Last Updated on 06.12.2018 16:11