Sequences from confirmation amplicon


Confirmation sequences for line 968B05 and hit At3g01600 are shown.
Line ID 968B05
Locus At3g01600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:230865
Primers used for PCR 73sm - 8474, show primer details
   
Sequence Name 53-K116491-0022-968-B05C-73sm
GenBank Accession LN508268 [GenBank]
Sequencing primer gene specific primer, 73sm: 5'-ATATGACTTCAGTTGCGGAGACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K116491-0022-968-B05C-73sm
TCAGCTTTTCCTTCTTCAGCGAAACGCCGTACTAGAAGAGCTTCTTTGTGTAGCATCAAG
AATCCAATCACAACCATCCAATCCAATTCAGCAAGCTCTCCTGAGAAGCCAACTGCAAAA
CCTCCATGACAACTAATTTAGAATCTTAAGTTCCATTGAATTTTCCGAAAGTAAACAAGG
TTTTTTTCAAGCAGGATCTTACAGTGAGGAAATCAGGGGGAGTCCCCAAATCTGCATTCT
CCAGATCTGATATCGAAAAACCATCGAAGTCCCCAACAGTTCCAAGGCTAGGATTGGGGA
GAACCTGATTTTCCCAAAGAGACGAAGCTTCCATGCTTTCGTCGCATATTAAGTTATCTT
CACTTAGGTTCACAACAAATTCCTCAAAAAATGGCTTAAACCGAAATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGT
TATTATAAT
   
Sequence Name 21-K116490-0022-968-B05C-8409
GenBank Accession LN495815 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K116490-0022-968-B05C-8409
GCATTTCATTGAATATATTTCGGTTTAAGCCATTTTTTGAGGAATTTGTTGTGAACCTAA
GTGAAGATAACTTAATATGCGACGAAAGCATGGAAGCTTCGTCTCTTTGGGAAAATCAGG
TTCTCCCCAATCCTAGCCTTGGAACTGTTGGGGACTTCGATGGTTTTTCGATATCAGATC
TGGAGAATGCAGATTTGGGGACTCCCCCTGATTTCCTCACTGTAAGATCCTGCTTGAAAA
AAACCTTGTTTACTTTCGGAAAATTCAATGGAACTTAAGATTCTAAATTAGTTGTCATGG
AGGTTTTGCAGTTGGCTTCTCAGGAGAGCTTGCTGAATTGGATTGGATGGTTGTGATTGG
ATTCTTGCTGCTACACAAAGAAGCTCTTCTAGTACGGCGTTTTCGCTGAAGAAGGAAAAA
GCTGGAATATGTCATAAATAAGAAAACTTTAAAGTCTCCGCAACTAAAGTCATAT

Last Updated on 06.12.2018 16:11