Sequences from confirmation amplicon


Confirmation sequences for line SALK_009022 and hit At1g14580 are shown.
Line ID SALK_009022
Locus At1g14580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4989899
Primers used for PCR 80sm - LBb3, show primer details
   
Sequence Name 35-K116652-SALK_009022C-80sm
GenBank Accession LN485223 [GenBank]
Sequencing primer gene specific primer, 80sm: 5'-CAAGAGAGAATTGTATGCAAGCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K116652-SALK_009022C-80sm
AATCTAAACAAAATTTTTTCCTTATCGGAAATGATTAGGGTTCTTATATTATTTTCGGCT
AAAATGGAAAATTCTTCACTTATTATTCATAAAAAAATTAAGTAAGAGGTACTTGCTTGG
GTTTCCGGGTTGGTTCCTTCGCTTCTTGGGTGGTGGAGCAACGGAGGAAGTGGGTTGTTG
TTGAACCATGGTCATCGCAGCCTCTTGACGGTTAAAGTTGTTTGGTCCGGGTCCGGCAGC
GGCACTTGAGAGGAGGAAGGTCGGAGTGGAGGACAAAGCAATTGTGTTGTACGATGAAGA
CATCTTCTCAGCTGGTCGGTTTTTGCTAAAGCTACAAAGATCCATAAAGAAAAAATAATT
TTAAAAAATGAGAGAGAAGATAAAAATCAAATATGATTCAGAAGAAAACGAAAGCGGGGA
ATTAGAAGAACAGGAAGAAGCCATAGAGATTGACGACATGAAAAAAAAAATTCAATGTAC
TGGGGTAAACAATATATCCAAATTGACGTCAATTATTTTGAAGGAGCCACTCAGCCGCGG
GTTCTCTTTGTTATCCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCC
   
Sequence Name 10-K116651-SALK_009022C-R210
GenBank Accession LN484562 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K116651-SALK_009022C-R210
ACCACAATATATCCTGGATAACAAGAGAACCCGCGGCTGAGTGGCTCCTTCAAAATAATT
GACGTCAATTTGGATATATTGTTTACCCCAGTACATTGAATTTTTTTTTTCATGTCGTCA
ATCTCTATGGCTTCTTCCTGTTCTTCTAATTCCCCGCTTTCGTTTTCTTCTGAATCATAT
TTGATTTTTATCTTCTCTCTCATTTTTTAAAATTATTTTTTCTTTATGGATCTTTGTAGC
TTTAGCAAAAACCGACCAGCTGAGAAGATGTCTTCATCGTACAACACAATTGCTTTGTCC
TCCACTCCGACCTTCCTCCTCTCAAGTGCCGCTGCCGGACCCGGACCAAACAACTTTAAC
CGTCAAGAGGCTGCGATGACCATGGTTCAACAACAACCCACTTCCTCCGTTGCTCCACCA
CCCAAGAAGCGAAGGAACCAACCCGGAAACCCAAGCAAGTACCTCTTACTTAATTTTTTT
ATGAATAATAAGTGAAGAATTTTCCATTTTAGCCGAAAATAATATAAGAACCCTAATCAT
TTCCAATAAGGAAAAAATTTTGTTTAGATTATATACCTTTTATTCGATCAATTTAATCAA
AGAAGATTAGTATTTGATGTGCTTGCATACAATTCCTCTC

Last Updated on 06.12.2018 16:11