Sequences from confirmation amplicon


Confirmation sequences for line SALK_010040c and hit At4g13640 are shown.
Line ID SALK_010040c
Locus At4g13640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7936907
Primers used for PCR 10ii - LBb3, show primer details
   
Sequence Name 27-K112623-SALK_010040C-10ii
GenBank Accession LN485062 [GenBank]
Sequencing primer gene specific primer, 10ii: 5'-CACAATCGTTACCACAGAGAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K112623-SALK_010040C-10ii
CTCCTTGCGATTTGATTAATTTTTAGTAAATTAGAGAATTGAAACTCACACGAAGAAGAA
GAAGTTGAGAAATCTTGGGGAAGCAAGCACAACGTAAACGGCTCTTCCTCTGCCGTTTTT
ATTTTCTCTCTTTGTTCAAAAATCAAAACTTCTTTTTTAGTTTTCAAAACTTCAGTTGCA
AATCACCGTACGAGTGAGCAGATACGCCGTCATTTTATCGCCGACCCCATCACTCTCTTC
CTCTTGAGATTTTTTTTTTTTTCTTGAACTTTTAATTTAACACCCTTAACCAATTTTGGG
CTTTTCCCCAAATGTGGGCCAACAATTTTGTAAACAAAATCCATTCTTTGGCAGGGAAAT
AATTTAGAATTTTTAAAGGAGTATTTTTTAGGTTAAACAATTACAAAATTTCTATTTTTG
GGTTTGCCCAAAAAAAAAAAAAAAGGGCAGCTTTAAAAAAATTTGAACCAAATCCCCAAC
CTAAAAAAGGGGCTCCGAATTCAACAAATTTTTCCTTAACCCCTAAAACAATTTGAATAA
CAAATTTTAAATTTAATTCCACCATGCCAAATTTAAATAGCCAAAAATCGGGTCCCGCTC
TCTCTCTTTTTTTGGCAAATTTCACCCAGGGCTACAACAAGGCAAACCCCACCCGGCACC
ATTGACCCTGGACCCTAAAACAATTAAAAAACACTTGGGGAAGTTTTTAAAGGAACGGGG
GGGGTTTTTCTTTTCCCCAGTGAAACGG
   
Sequence Name 03-K112622-SALK_010040C-R210
GenBank Accession LN484339 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K112622-SALK_010040C-R210
GCGTCATGATGCTGGATGGGTTATGCCTAGTAGTAGCATTGGATGAAGTTAGCAAGAGAG
AGAGAGAGAGCGAGACCAGATTTTAGCTTATTTAATTTTGCTATGTTGTATTTAACTCTA
AACTCTGTCATCAAACTTGTCTTAGCGTCTATGTATATATTTGTTGAATCTGTAGCCTCT
CTTTTAGTTTGTGTATTTGCTTCAACTTTTTCTAAGCTTGCCTTCTTTTTTCTTTTTTGG
ACAAACCTAAATATAGAATTTATGTAATTGTATAACATAAAAAATACTCATTAAATACTC
ATAACTTATCTCCATGCTATAGATTGGATCTTGTCTACAAATTAGTTGGCACACATTTGC
GTAAATGCACAAATTTGATTACGTGTGTAAAATAAAATGTCAAAGAAAAAAAAAAAATTC
TCAAAAGAAAAAAAGTGAGGGGGTCGGCAAAAAAAGGACGGCTTATCTGCCCCCCCGAAG
GGGGATTTGCAACTGAATTTTTAAAAACAAAAAAAAAATTTTTATTTTTTGACCAAAGAG
AAAAAATAAAACCGGCAAAGAAAAACCCTTTTACTTTGGGCTTGCTCCCCAAAAATTTCC
AAACTTCTTCTTCTTGGGGGGATTTTCATTTCCCAATTTTACAAAAAATAAACCAATTCC
CAGGGATTTTCTCTGTGTAAACAATGGATCCACCGTTAATTTTTCTCGGGGGAAACGAAT
GGGG

Last Updated on 06.12.2018 16:11