Sequences from confirmation amplicon


Confirmation sequences for line SALK_014446c and hit At3g07310 are shown.
Line ID SALK_014446c
Locus At3g07310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2329675
Primers used for PCR 27mv - LBb3, show primer details
   
Sequence Name 21-K114624-SALK_014446C-27mv
GenBank Accession LN484886 [GenBank]
Sequencing primer gene specific primer, 27mv: 5'-TCAGAGAGTTTAACACAGGCAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K114624-SALK_014446C-27mv
TACGAACTAGCACAATGAAATGTAAAAAGCAAAACCAGCCCTTATCAAACACTAAAACAA
AACTTGCTTCACAAGGCAGAGTATGAGAAAGCACCTGAAGATCATAACTACAGAATTCTC
TTGTTTCAATTCAACCATATCATTAGTGTGATTCTCAAATCATCATAAACTGATAAACAC
CATCAAACAACTTATAATCAAACTTTACAAGCTTATGTGATAAATACTAATAAAATACTG
ATTTGTGGTCGATTAAATAAGAGTATGTAAGTAATGATTTACACATATTTCTTTGTATGT
TTATAATTCTACAGCTGTTTACGGATCTTTGAATTGTGAGAGATTAGATAAGGAAAGGTT
TCACGGACCTGTGGAGAGAAGCCTCATCGGAACCAGAGCTGAGAGACATACGAGCTAAAG
CAGCTTCCCTATCATCAGCCAGTTGCTTTAGCTCATCAGCGGCGGCGTAATGAAATAGCT
GCCGTTTGTTTAGCAACACGCTTCTCAGAAACCTCCCCTGCGCCGATCGTGGCTCAAGCG
GCGCGTTTAGACTGCTTCCAGGCTCACACCGACTCTGTCCCGCCGCCGCAACCACAACCA
TCGAACTATACTGGATCTTCCTCTTCGCCGGAATAACAAAACCACCGCCTCCATCGCCGC
TCTGTCTCCTGGAACGCGACGGGAGAAACTGAAGCGCCGTTTGATTAGAGAGACAACTAT
CCCTAANAACAGTGGACAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTT
TAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTC
ACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCANGCGA
AAATCCTGTTTGATGGTGGTT
   
Sequence Name 05-K114623-SALK_014446C-R210
GenBank Accession LN484406 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K114623-SALK_014446C-R210
TTTAGGGATAGTTGTCTCTCTAACAAACGGCGCTTCAGTTTCTCCCGTCGCGTTCCAGGA
GACAGAGCGGCGATGGAGGCGGTGGTTTTGTTATTCCGGCGAAGAGGAAGATCCAGTATA
GTTCGATGGTTGTGGTTGCGGCGGCGGGACAGAGTCGGTGTGAGCCTGGAAGCAGTCTAA
ACGCGCCGCTTGAGCCACGATCGGCGCAGGGGAGGTTTCTGAGAAGCGTGTTGCTAAACA
AACGGCAGCTATTTCATTACGCCGCCGCTGATGAGCTAAAGCAACTGGCTGATGATAGGG
AAGCTGCTTTAGCTCGTATGTCTCTCAGCTCTGGTTCCGATGAGGCTTCTCTCCACAGGT
CCGTGAAACCTTTCCTTATCTAATCTCTCACAATTCAAAGATCCGTAAACAGCTGTAGAA
TTATAAACATACAAAGAAATATGTGTAAATCATTACTTACATACTCTTATTTAATCGACC
ACAAATCAGTATTTTATTAGTATTTATCACATAAGCTTGTAAAGTTTGATTATAAGTTGT
TTGATGGTGTTTATCAGTTTATGATGATTTGAGAATCACACTAATGATATGGTTGAATTG
AAACAAGAGAATTCTGTAGTTATGATCTTCAGGTGCTTTCTCATACTCTGCCTTGTGAAG
CAAGTTTTGTTTTAGTGTTTGATAAGGGCTGGTTTTGCTTTTTTACATTTCATTGTTGCT
TAGTTCGTTAAATAGTTTCCGTATATNTTAGTTTCTTGCCTGTGTT

Last Updated on 06.12.2018 16:11