Sequences from confirmation amplicon


Confirmation sequences for line SALK_016439c and hit At4g21745 are shown.
Line ID SALK_016439c
Locus At4g21745 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11549659
Primers used for PCR 10va - LBb3, show primer details
   
Sequence Name 30-K117581-SALK_016439C-10va
GenBank Accession LN485137 [GenBank]
Sequencing primer gene specific primer, 10va: 5'-CCTACTGGTATAGGAGGCACATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K117581-SALK_016439C-10va
TCGATTCTGTAGTTAACGAAGATAAACAGTTCCATTTAGTATGTAACTCTAACTAAAAAA
TATTTATGCACTCTATTAATTTAGTGCACGCAGCAATAAAATTACCAGTGGAATCAGAAG
TGGTGAAGAAAGAATCTGAATGTCTTGTATGACTGAAAGATGATGTTCTTGGAGATAAAA
ACTCTGCTCCTGCCTTAAAGTCACTCATCTACACCCCGAAAAACAAATAAATTTTGAAAT
AAGCATCATCAGAAAACTAGATTATCAGTATTGTGTGTGTTAAAACATCATTTGATACTT
CAAGAGTTCATATTCATAACATACTTTTAAATGTATAGTTCAGACTTGAAAAGTATAACA
AGAAATATTAATATTTAGATAAAGAGAGAGAGCTTACCCAACCAGGATCACTGCCAGAGG
AGCCCCAACCAATATGGGCCACATGTTTTACTTCTGTTGGGTGCCCAATTTCCAAATCTC
TTTCTTTCCCAACTGTTGACCAAAAAAAATTCATCATTTAATAGCTGTTCTTGTAATACT
TAGAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGG
GGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCC
CTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTT
GATGGTGGTTC
   
Sequence Name 14-K117580-SALK_016439C-R210
GenBank Accession LN484679 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117580-SALK_016439C-R210
TTTGTTCAAGTATTACAAGAACAGCTATTAAATGATGAATTTTTTTTGGTCAACAGTTGG
GAAAGAAAGAGATTTGGAAATTGGGCACCCAACAGAAGTAAAACATGTGGCCCATATTGG
TTGGGGCTCCTCTGGCAGTGATCCTGGTTGGGTAAGCTCTCTCTCTTTATCTAAATATTA
ATATTTCTTGTTATACTTTTCAAGTCTGAACTATACATTTAAAAGTATGTTATGAATATG
AACTCTTGAAGTATCAAATGATGTTTTAACACACACAATACTGATAATCTAGTTTTCTGA
TGATGCTTATTTCAAAATTTATTTGTTTTTCGGGGTGTAGATGAGTGACTTTAAGGCAGG
AGCAGAGTTTTTATCTCCAAGAACATCATCTTTCAGTCATACAAGACATTCAGATTCTTT
CTTCACCACTTCTGATTCCACTGGTAATTTTATTGCTGCGTGCACTAAATTAATAGAGTG
CATAAATATTTTTTAGTTAGAGTTACATACTAAATGGAACTGTTTATCTTCGTTAAACTA
CAGAATTCGATCAGGACCAACTGAATATATCAGATAGGATAAGAGATGTGCCTCC

Last Updated on 06.12.2018 16:11