Sequences from confirmation amplicon


Confirmation sequences for line SALK_016556c and hit At1g70440 are shown.
Line ID SALK_016556c
Locus At1g70440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26550603
Primers used for PCR 14ty - LBb3, show primer details
   
Sequence Name 24-K117180-SALK_016556C-14ty
GenBank Accession LN484984 [GenBank]
Sequencing primer gene specific primer, 14ty: 5'-GGTAAGATTGTTTAGAACCGGTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K117180-SALK_016556C-14ty
GATCCCGGCATAGAGAATATTCTTGATCCCTTCTTCTCACCTTCACCAACTAAAGCCCTG
AAACCATCATCAATTTTCACTTTTTCATCATCAAAATCACAAACTAAGAACTTAGACAAC
TGAGAAAAAACATAACACTTACGCAGCGAGAGAGTAACGATGATGAACAAGATGAATTCC
AACACCGTGAGATCCGACGTCGTTTTCGACTTTCTTGATCTCTCTATTACTAAACCCATA
CGTAATAACACGATCGATCTCTTCTTTAGAACCAGAGTACCAACCGTACCTAATATTCGC
GTAACCGTTGTTCTTCCTCTTCATAGCTTCTGTGAAAAGCTTGAACACAGCGAATTTAGC
TTTCGTTGTGATTAATCTCTGCGTTAAGATTTTTCGTACGGTGACGATTGTTGTTTCGGT
GGCGAATGAGCCCATTCCGGAGAGGAAACAGTTTTTGATGAGGTTGTGTTCGAAGGTGGC
TTCACGGAGAAGAATCGTTGCATCGCCGGAGAAGTTAGGTGCGTTGTCGGAGATGGGGTT
GATGATTTCGCCGTTGTCTAAATTCGTGACGGATTCTTGATCTTCGATCTCGACTTGTGC
CGCCATTAGAAAAGAAGAAAGAGAGTTTCTTGAGAAAAGAGAAAGTGGAGAAAGGTTTGT
TTTCAATGGCGGTTTTAGGGATTTGCAGTGATATTGTGGTGTAAACAAATTGACGCTTAG
ACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCA
GTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGC
GGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 03-K117179-SALK_016556C-R210
GenBank Accession LN484349 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117179-SALK_016556C-R210
CCACAATATCACTGCAAATCCCTAAAACCGCCATTGAAAACAAACCTTTCTCCACTTTCT
CTTTTCTCAAGAAACTCTCTTTCTTCTTTTCTAATGGCGGCACAAGTCGAGATCGAAGAT
CAAGAATCCGTCACGAATTTAGACAACGGCGAAATCATCAACCCCATCTCCGACAACGCA
CCTAACTTCTCCGGCGATGCAACGATTCTTCTCCGTGAAGCCACCTTCGAACACAACCTC
ATCAAAAACTGTTTCCTCTCCGGAATGGGCTCATTCGCCACCGAAACAACAATCGTCACC
GTACGAAAAATCTTAACGCAGAGATTAATCACAACGAAAGCTAAATTCGCTGTGTTCAAG
CTTTTCACAGAAGCTATGAAGAGGAAGAACAACGGTTACGCGAATATTAGGTACGGTTGG
TACTCTGGTTCTAAAGAAGAGATCGATCGTGTTATTACGTATGGGTTTAGTAATAGAGAG
ATCAAGAAAGTCGAAAACGACGTCGGATCTCACGGTGTTGGAATTCATCTTGTTCATCAT
CGTTACTCTCTCGCTGCGTAAGTGTTATGTTTTTTCTCAGTTGTCTAAGTTCTTAGTTTG
TGATTTTGATGATGAAAAAGTGAAAATTGATGATGGTTTCAGGGCTTTAGTTGGTGAAGG
TGATGAAGAAGGGATCAAGAATATTCTCTTATGCCGTGTGATTCTTGGTAAACCGGAACA
AATTGTGACCGGTTCTAAAAAATCTTACC

Last Updated on 06.12.2018 16:11