Sequences from confirmation amplicon


Confirmation sequences for line SALK_017597c and hit At2g32930 are shown.
Line ID SALK_017597c
Locus At2g32930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13968061
Primers used for PCR 17ht - LBb3, show primer details
   
Sequence Name 75-K112669-SALK_017597C-17ht
GenBank Accession LN486129 [GenBank]
Sequencing primer gene specific primer, 17ht: 5'-ACGAGTTTTGATTTTGTGTCTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K112669-SALK_017597C-17ht
GAACTCGCAGCAGGTTCAGAACTCTACTGGGTCGATTCGATCTCCCGACAAAATTGAAGG
TAGTTACTCTCTTTTTTTTCATTGAAGATCCAACGAAACGGGTTTTTTCCGTAATGTGTT
TGAAGATCATATAATTTCTGTTGTTGTTTTTGATCATTCAAGTCTTATTGTGATTTCGTT
TATTTTTTCTGAGAGTTCTGATCATTCAAGTCTTAAAAGTTCGAAAATTTAGAGTTTTGT
CTTTTTGGAGTTATCTTGTTGGATTCGTCTTGTCGGATCATTGATTCCGAATGCTTCTTC
TACATTTATGGAAGTTAAAGATCATGTTTTTTTTCATTGTGTGATATTGTTATATTGTGG
TGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTG
GGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGC
CCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTT
TGATGGTGGTTCCG
   
Sequence Name 65-K112668-SALK_017597C-R210
GenBank Accession LN485873 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K112668-SALK_017597C-R210
TTTGTTACCCACAATATAACAATATCACACAATGAAAAAAAACATGATCTTTAACTTCCA
TAAATGTAGAAGAAGCATTCGGAATCAATGATCCGACAAGACGAATCCAACAAGATAACT
CCAAAAAGACAAAACTCTAAATTTTCGAACTTTTAAGACTTGAATGATCAGAACTCTCAG
AAAAAATAAACGAAATCACAATAAGACTTGAATGATCAAAAACAACAACAGAAATTATAT
GATCTTCAAACACATTACGGAAAAAACCCGTTTCGTTGGATCTTCAATGAAAAAAAAGAG
AGTAACTACCTTCAATTTTGTCGGGAGATCGAATCGACCCAGTAGAGTTCTGAACCTGCT
GCTGAGTTTCCGACATGGCTCGTTATGAAACAAACACAGTCACAGACACAAAATCAAAAC
TCG

Last Updated on 06.12.2018 16:11