Sequences from confirmation amplicon


Confirmation sequences for line SALK_017723 and hit At5g11930 are shown.
Line ID SALK_017723
Locus At5g11930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3845490
Primers used for PCR CG34 - R212, show primer details
   
Sequence Name 62-K107135-SALK_017723C-CG34
GenBank Accession LN485784 [GenBank]
Sequencing primer gene specific primer, CG34: 5'-GATGCCCATAACCATTATATGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K107135-SALK_017723C-CG34
ATTTAGATTTAGACCAGCTATATGGTTGTTAAAGAAAACAAGACCAAGCTACAGTATATA
AGTCATAAATAAGAAGCGACCAAGGTGACCGTCGAAAACAAATTTATTAAAGTGACATAT
AAATTAAAAACAAATAAAATAATTAATTAGCTAATGGTTACGTCATTATAGTGACACCCA
ATTTCTCCATTTATGGTCGCTCTCATCTCGCGTTGACATCAACAAAAACTCCCACTCTCT
CTCTCCCTCTCTAAGTACTCTTTCTCTCTAATGAAGACGATGCGAGGTTTACGAAACTGC
TCAAACGACGCCGTAACGCTAGACCTGACGGTTCATCCTCCTCCTCCTCCTCCTCTTCCT
CCTTCAAACACTGATAGTTTAAACTGAAGGCGGGAAACGACAATCTGATCATGAGCGGAG
AATTAAGGGAGTCACGTTATGACCCCCGCCGATGACGCGGGACAAGCCGTTTTACGTTTG
GAACTGACAGAACCGCAACGTTGAAGGAGCCACTCAGCCGCGGGTTTCTGGAGTTTAATG
AGCTAAGCACATACGTCAGAAACCATTATTGCGCGTTCAAA
   
Sequence Name 54-K107134-SALK_017723C-R214
GenBank Accession LN485596 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K107134-SALK_017723C-R214
GCGGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCCGC
CTTCAGTTTAAACTATCAGTGTTTGAAGGAGGAAGAGGAGGAGGAGGAGGAGGATGAACC
GTCAGGTCTAGCGTTACGGCGTCGTTTGAGCAGTTTCGTAAACCTCGCATCGTCTTCATT
AGAGAGAAAGAGTACTTAGAGAGGGAGAGAGAGAGTGGGAGTTTTTGTTGATGTCAACGC
GAGATGAGAGCGACCATAAATGGAGAAATTGGGTGTCACTATAATGACGTAACCATTAGC
TAATTAATTATTTTATTTGTTTTTAATTTATATGTCACTTTAATAAATTTGTTTTCGACG
GTCACCTTGGTCGCTTCTTATTTATGACTTATATACTGTAGCTTGGTCTTGTTTTCTTTA
ACAACCATATAGCTTGGTCTAAAATTCTAAATTATAACTTAATATATATCGAAAATGGAC
ATATAATGGTTAGTGGGCAT
Primers used for PCR CG33 - R204, show primer details
   
Sequence Name 63-K107135-SALK_017723C-CG33
GenBank Accession LN485812 [GenBank]
Sequencing primer gene specific primer, CG33: 5'-CTTACCGACTGGCAAAAGATAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K107135-SALK_017723C-CG33
TGGATTATGGAAAATTTTACAATGGATTTTATTATCAGAATTTCTTAAGGTTATAAATAG
GCTAGACTTGAAAGAAAGAAAGAAAAATACAGAAAATTACAATTATGCCCATAAGGCTCC
AACCTCGACGAGTCTAGGAATAAGCTGACCACTTAGGTGAAGTGCTACAAGTGACTCGAA
GCCACCGACGCAAGTACCACCGATGAAAAGCACCGGAGCGGCTTCAACGGCGAGGTAAGC
AATTTCTCCGTCGTCGATCTCGATCACTGTTGGGTGAACTCCAACGGTCGATAGAAGCTT
CTTCATGACGTGGCACATGCAACATGAGGAAAATCGAGTGAATATGATGACTGGATGCTC
TGATATCAGCCGTCCGATCTTTGACTCTGATGTTTCCTCTTCATCGAACGACAGGCTCGT
CGAGGCGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTA
ATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACG
   
Sequence Name 55-K107134-SALK_017723C-R210
GenBank Accession LN485616 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K107134-SALK_017723C-R210
GTCATTTGTTTACCCGCCTCGACGAGCCTGTCGTTCGATGAAGAGGAAACATCAGAGTCA
AAGATCGGACGGCTGATATCAGAGCATCCAGTCATCATATTCACTCGATTTTCCTCATGT
TGCATGTGCCACGTCATGAAGAAGCTTCTATCGACCGTTGGAGTTCACCCAACAGTGATC
GAGATCGACGACGGAGAAATTGCTTACCTCGCCGTTGAAGCCGCTCCGGTGCTTTTCATC
GGTGGTACTTGCGTCGGTGGCTTCGAGTCACTTGTAGCACTTCACCTAAGTGGTCAGCTT
ATTCCTAGACTCGTCGAGGTTGGAGCCTTATGGGCATAATTGTAATTTTCTGTATTTTTC
TTTCTTTCTTTCAAGTCTAGCCTATTTATAACCTTAAGAAATTCTGATAATAAAATCCAT
TGTAAAATTTTCCATTAATCCACTCTTTCTTTCTGAAACACAAAAATGTTTTTTTTTCTT
TATCTTTTGCCAGTCGGTAA

Last Updated on 06.12.2018 16:11