Sequences from confirmation amplicon


Confirmation sequences for line SALK_020806c and hit At2g03450 are shown.
Line ID SALK_020806c
Locus At2g03450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1043328
Primers used for PCR BI36 - R204, show primer details
   
Sequence Name 05-K107118-SALK_020806C-R210
GenBank Accession LN484390 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K107118-SALK_020806C-R210
TCTCACAACAGTCATGGTTTCCCACATAAGAAAGAGTAAGTCTTTCCTTATTAGCAACCA
AACGAATGTACCCAAACTCGCCTCCACGGTACATTGAGTTAGCAGGCTGAGGAAAGATCG
GGACATCCTCGTGATTAGCTCTCGGTTCCCACATCGGTTGTGAGTCTTTTCCAGCCATAC
CAATCACAAGATGAACTGGATTTCCTTGCCAACGTTCACCACAAGTATTGTTACTAATCG
CACAAAACCTTTCATATCTATGTACATGTCCCCATAAAGCAACCGTCACATTGTTCTTCA
CTAACAACGGTTCCAAATGCTCGATCATCTTCTCTCTTATAGCAGCGTCTCTGATCTTCC
TACTCGTAGTGTACATAGGTCTATGCCCTTGGACAACAACAAACGGTGTCTTACTCCTAT
TAACAGACTCCAAATCACTCTTCAAAAAACTATACTGCTTCCCACCTTTAAGAAAATCAG
TCTCTGTCGAAATATAAACGAAATGAACAGAACCCATATCATAAGAATAGTAAAGGTTCC
GACTTTGAGGTCCCTTAACCATACCAGTAGCTTCCGTTGAATTACCAGGCATGTTGAACT
TAACACTATACGGTACACCACATTCACCACCACTATCTTTACCATAAACATAAGCAGCCC
AATCTGGTTTCCAAGGCTGGTTAGGCCAATCATACTCATGGTTACCAATACATACATGGT
AAGGCACTTTAGAAGCAATAGGCTCAATCTGAGTAAAGAACTCATCCCAAATCCACGAGT
AACCTCGTGCATAACTTATATCTCCGATATGTGATACAATCACAGGCTTATCATCACCTA
AAGCTTCAATGTCTCTTAAAATCCATTTCACAGTTGATAAACTTTCTTCTTCTCCACGGA
TAAATGTTGTATAAGGTGTATAACATCCCATATCACCAAACATGAAAGCTAATGTTTCTT
CTGAACCCTCATTTCGAGAAACAAGCTA
   
Sequence Name 21-K107119-SALK_020806C-BI36
GenBank Accession LN484874 [GenBank]
Sequencing primer gene specific primer, BI36: 5'-AGGTTGGGAGTGATTTAAAAGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K107119-SALK_020806C-BI36
TGAGGTTCGAAGAACATTAGCTTTCATGTTTGGTGATATGGGATGTTATACACCTTATAC
AACATTTATCCGTGGAGAAGAAGAAAGTTTATCAACTGTGAAATGGATTTTAAGAGACAT
TGAAGCTTTAGGTGATGATAAGCCTGTGATTGTATCACATATCGGAGATATAAGTTATGC
ACGAGGTTACTCGTGGATTTGGGATGAGTTCTTTACTCAGATTGAGCCTATTGCTTCTAA
AGTGCCTTACCATGTATGTATTGGTAACCATGAGTATGATTGGCCTAACCAGCCTTGGAA
ACCAGATTGGGCTGCTTATGTTTATGGTAAAGATAGTGGTGGTGAATGTGGTGTACCGTA
TAGTGTTAAGTTCAACATGCCTGGTAATTCAACGGAAGCTACTGGTATGGTTAAGGGACC
TCAAAGTCGGAACCTTTACTATTCTTATGATATGGGTTCTGTTCATTTCGTTTATATTTC
GACAGAGACTGATTTTCTTAAAGGTGGGAAGCAGTATAGTTTTTTGAAGAGTGATTTGGA
GTCTGTTAATAGGAGTAAGACACCGTTTGTTGTTGTCCAAGGGCATAGACCTATGTACAC
TACGAGTAGGAAGATCAGAGACGCTGCTATAAGAGAGAAGATGATCGAGCATTTGGAACC
GTTGTTAGTGAAGAACAATGTGACGGTTGCTTTATGGGGACATGTACATAGATATGAAAG
GTTTTGTGCGATTAGTAACAATACTTGTGGTGAACGTTGGCAAGGAAATCCAGTTCATCT
TGTGATTGGTATGGCTGGAAAAGACTCACAACCGATGTGGGAACCGAGAGCTAATCACGA
GGATGTCCCGATCTTTCCTCAGCCTGCTAACTCAATGTACCGTGGAGGCGAGTTTGGGTA
C
Primers used for PCR 49xh - LBb3, show primer details
   
Sequence Name 19-K119050-SALK_020806C-49xh
GenBank Accession LN484837 [GenBank]
Sequencing primer gene specific primer, 49xh: 5'-ATGTGGTGCCTGTAGGTCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K119050-SALK_020806C-49xh
TTGGCCAACAATGGATCATTATAAGTTTTCAGCTTCTTGCGAGATACAGAATGTTTTGTA
CTCTTGTACTCATGGAGTGTTCAGATGGTATGACACTAACGCAATGATGTGGAGAGATTT
GAAGGGTTTGGTAGGACTACCTATGTTCGGTTCTGGTGCCAATGTTAAGTTGGCTGATTA
TGGAGGAAAATTAACGGTTTTGTGGGAGGAGGAGTTGCCTTCTCGTGGACCTGATAGCGG
CTATAAGAAGATGATTCGGTGTGCAGAGATTGCGCTTGAAAGGCGCAAAAGTTGTGAAAT
TTGGGGGATTGTTGAGTGGTTTGGTGATGTGCTTACCGAACCTGTAGGATATGTTGTTTT
GGAGAAGGTTCTTGATGTTACTCTTTGATGAAACTTGAATGTGTTTTCTTTTGGAATACT
TGTGTGTTCTTTTGGTGAATGTAACTTCTGATGAATGAGTCTTGTCTCTTGTTTCACTTC
TAATAATTTGATATACAAATCTGTGTGGCAAACAAACAAACAAACAAACAAAAACAAATC
AGTTGAATCATGTCTCCTCGTTTTTCACTTGGATCCAACTACGATTAGATGATCCAACTC
CAGATTCTTTCTTTTTACGACTAAGAAAACCGACAAAGTACCCAAAAATCACTCCCACAA
CCATCACACTTGCTCCTTCAATGTTCCACAAGACTGCA
   
Sequence Name 03-K119049-SALK_020806C-R210
GenBank Accession LN484356 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K119049-SALK_020806C-R210
GTCATTTGTTTCCCACAATATATCCTGGGGAAGAGTAATGATGTTGTTGAGATTTTGGCT
TCTGGGGAAGTTATTAGCGGTAGTGATGATGGTACTAAAGACTCAAACTTTGGATCAGAA
TCTGACTTTGCAGTCTTGTGGTACATTGAAGGAGCAAGTGTGATGGTTGTGGGAGTGATT
TTTGGGTACTTTGTCGGTTTTCTTAGTCGTAAAAAGAAAGAATCTGGAGTTGGATCATCT
AATCGTAGTTGGATCCAAGTGAAAAACGAGGAGACATGATTCAACTGATTTGTTTTTGTT
TGTTTGTTTGTTTGTTTGCCACACAGATTTGTATATCAAATTATTAGAAGTGAAACAAGA
GACAAGACTCATTCATCAGAAGTTACATTCACCAAAAGAACACACAAGTATTCCAAAAGA
AAACACATTCAAGTTTCATCAAAGAGTAACATCAAGAACCTTCTCCAAAACAACATATCC
TACAGGTTCGGTAAGCACATCACCAAACCACTCAACAATCCCCCAAATTTCACAACTTTT
GCGCCTTTCAAGCGCAATCTCTGCACACCGAATCATCTTCTTATAGCCGCTATCAGGTCC
ACGAGAAGGCAACTCCTCCTCCCACAAAACCGTTAATTTTCCTCCATAATCAGCCAACTT
AACATTGGCACCAGAACCGAACATAGGTAGTCCTACCAAACCCTTCAAATCTCTCCACAT
CATTGCGTTAGTGTCATACCATCTGAACACTCCCTGAGTACAAGAGTACAAAACCTTCTG
TATCTCGCAAGAAGCTGAAAACT

Last Updated on 06.12.2018 16:11