Sequences from confirmation amplicon


Confirmation sequences for line SALK_020960c and hit At4g18640 are shown.
Line ID SALK_020960c
Locus At4g18640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10261734
Primers used for PCR 03as - R204, show primer details
   
Sequence Name 11-K107313-SALK_020960C-03as
GenBank Accession LN484573 [GenBank]
Sequencing primer gene specific primer, 03as: 5'-TTGGTACCCCTACATTTTGTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K107313-SALK_020960C-03as
CTAAGAACGAGTAAAATGGAAACAAAGAGAAATAAGATTTTTTACCGGTAACAAAAGCTT
TCTGCAATTGTCCACTAAGACCAGTTTTCCAAGGACCTATGCTCTTGACAGCTCTTTTCC
GGCAGAAGAAGATAACCGCTACTATAATCAGTAAACCGACGAAAGAAGCAACCGCGATAA
CCACATACAGCCATACATGTTTTGATCCTTTTGACTGTTTATTATCTTGTGGCTGATTCT
TAGAGATATCAGGACTAGTACTGCCCTTATCAGGAGAAGGTACAGGAGGGACTAAAGGTG
GTCTTCTCTTCTTTGCATTGGTTACTGCAGGGAAAGACCCGCTGCTTCGAGGTGAAGCTT
CAGTTATAATCCCGGGAGAAGGACTAGGAGCTGAAGGAGCAGGCTCAGCCGCTAAATTAC
TTGTCTCCTCGAGTAGCTCACATTGCGGTGACAACAATATATTGTGGTGTAAACAAATTG
ACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCT
TTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTG
CAGCAAGCGGNTCCACG
   
Sequence Name 51-K107289-SALK_020960C-R210
GenBank Accession LN485527 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K107289-SALK_020960C-R210
GTGCTAGCGTCATTTGTTTACCCCAATATATTGTTGTCACCGCAATGTGAGCTACTCGAG
GAGACAAGTAATTTAGCGGCTGAGCCTGCTCCTTCAGCTCCTAGTCCTTCTCCCGGGATT
ATAACTGAAGCTTCACCTCGAAGCAGCGGGTCTTTCCCTGCAGTAACCAATGCAAAGAAG
AGAAGACCACCTTTAGTCCCTCCTGTACCTTCTCCTGATAAGGGCAGTACTAGTCCTGAT
ATCTCTAAGAATCAGCCACAAGATAATAAACAGTCAAAAGGATCAAAACATGTATGGCTG
TATGTGGTTATCGCGGTTGCTTCTTTCGTCGGTTTACTGATTATAGTAGCGGTTATCTTC
TTCTGCCGGAAAAGAGCTGTCAAGAGCATAGGTCCTTGGAAAACTGGTCTTAGTGGACAA
TTGCAGAAAGCTTTTGTTACCGGTAAAAAATCTTATTTCTCTTTGTTTCCATTTTTACTC
TGTTTCTTTAGGAACATTAACACTGAAACTGAGAACAAAATGTAGGGGTACCA

Last Updated on 06.12.2018 16:11