Sequences from confirmation amplicon


Confirmation sequences for line SALK_024560c and hit At5g06220 are shown.
Line ID SALK_024560c
Locus At5g06220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1880548
Primers used for PCR 36wm - LBb3, show primer details
   
Sequence Name 36-K118709-SALK_024560C-36wm
GenBank Accession LN485244 [GenBank]
Sequencing primer gene specific primer, 36wm: 5'-CTTGTACCGTCAAGGTAAACCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K118709-SALK_024560C-36wm
ATTTATGCTCTTTCGTGGATTTTTCTGATTAATCGTGGTATTGTAGCTGTTCAACCCTTG
GATGTCCGTTGGAACCTTGATTTCGTGCAGAAGAGTGCTACAATTGGATTATATATCAAA
CTGCTGCGGAAATTCCAGGACTCAATTGTTTGTGACCTATGATGATAAGAACTACTTTCT
TCAGAGAAAACTTTTTGGAAACAAGAAAATGAGTTCACGGAGGACTACCCAGCCTTTTCT
CCTTGCATCTGCTGAAGATGGAGTGGCTGTTAATGGGTCAAGAGCTAGCGATGATGTTCA
GGAGATGAGGGCCAAACTCAGTGGATCACTACAAGATGAATATAGCTGTGGTGAACTTAT
TCAGTCTTTGCATGATGCTGCCAGGACTTTTGAACTGGCTCTTAAAGAGAAGATATCTTC
AAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGT
TTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGA
GAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGT
GGTACCGAATCGGGCAAAA
   
Sequence Name 11-K118708-SALK_024560C-R210
GenBank Accession LN484598 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K118708-SALK_024560C-R210
GTCATTTGAGATTCTTCTCTTTAAGAGCCAGTTCAAAGTCCTGGCAGCATCATGCAAAGA
CTGAATAAGTTCACCACAGCTATATTCATCTTGTAGTGATCCACTGAGTTTGGCCCTCAT
CTCCTGAACATCATCGCTAGCTCTTGACCCATTAACAGCCACTCCATCTTCAGCAGATGC
AAGGAGAAAAGGCTGGGTAGTCCTCCGTGAACTCATTTTCTTGTTTCCAAAAAGTTTTCT
CTGAAGAAAGTAGTTCTTATCATCATAGGTCACAAACAATTGAGTCCTGGAATTTCCGCA
GCAGTTTGATATATAATCCAATTGTAGCACTCTTCTGCACGAAATCAAGGTTCCAACGGA
CATCCAAGGGTTTGAACAGCTACAATACCACGATTAATCAGAAAAATTCCACGAAAAGAG
CATAAAATCGAAGATTATCGAGAGAAATGTAAGCTATTGGTTTACCTTGAGGGTACAA

Last Updated on 06.12.2018 16:11