Sequences from confirmation amplicon


Confirmation sequences for line SALK_027039c and hit At3g17240 are shown.
Line ID SALK_027039c
Locus At3g17240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5891059
Primers used for PCR 20md - LBb3, show primer details
   
Sequence Name 80-K114279-SALK_027039C-20md
GenBank Accession LN486220 [GenBank]
Sequencing primer gene specific primer, 20md: 5'-CAACTTCAGGGTACGTGTAGACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K114279-SALK_027039C-20md
ACTCAACACATGCAACACCATCTTCTTCGGCTTTGTGAGCCAGCATTGGTCCTGGAATCA
CATCTCCAATTGCATAAACGCCTGAAACATTTGTCGAGAATCTCTCGTTCACCAGAATTC
TCCCGCCTTTGTCTGTCTCAACTCCGATTTTCTCTAGATCAAGTCCAGATGTGAACGGAG
TTCTACCAGCTGAGACGAGGACCACATCAGCTTCTAGAGTGGTCTGTTCTCCACCTTCAG
CAGGTTCCACTATGAGTTTAACACCATCTCCAGATGAATCTACCCCAACGACTTTAGTTT
TGAGCATAAATTTCATCTTCTGCTTCTCTAGTGAGCGTTGAAACTGCTTGCGGATTTCAC
CATCCATTGCTGGTACGATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAAC
ACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAA
CAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGT
TTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 66-K114278-SALK_027039C-R210
GenBank Accession LN485905 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K114278-SALK_027039C-R210
ACCCACAATATCGTACCAGCAATGGATGGTGAAATCCGCAAGCAGTTTCAACGCTCACTA
GAGAAGCAGAAGATGAAATTTATGCTCAAAACTAAAGTCGTTGGGGTAGATTCATCTGGA
GATGGTGTTAAACTCATAGTGGAACCTGCTGAAGGTGGAGAACAGACCACTCTAGAAGCT
GATGTGGTCCTCGTCTCAGCTGGTAGAACTCCGTTCACATCTGGACTTGATCTAGAGAAA
ATCGGAGTTGAGACAGACAAAGGCGGGAGAATTCTGGTGAACGAGAGATTCTCGACAAAT
GTTTCAGGCGTTTATGCAATTGGAGATGTGATTCCAGGACCAATGCTGGCTCACAAAGCC
GAAGAAGATGGTGTTGCATGTGTTGAGTTTATAGCAGGCAAACACGGGCATGTGGATTAC
GACAAAGTCCCTGGGGTTGTCTACACGTACCCCTGAAGTTG

Last Updated on 06.12.2018 16:11